Repairing damage in the craniofacial skeleton is challenging. Craniofacial bones require intramembranous ossification to generate tissue-engineered bone grafts via angiogenesis and osteogenesis. Here, we designed a mineralized collagen delivery system for BMP-2 and vascular endothelial growth factor (VEGF) for implantation into animal models of mandibular defects. BMP-2/VEGF were mixed with mineralized collagen which was implanted into the rabbit mandibular. Animals were divided into (i) controls with no growth factors; (ii) BMP-2 alone; or (iii) BMP-2 and VEGF combined. CT and hisomputed tomography and histological staining were performed to assess bone repair. New bone formation was higher in BMP-2 and BMP-2-VEGF groups in which angiogenesis and osteogenesis were enhanced. This highlights the use of mineralized collagen with BMP-2/VEGF as an effective alternative for bone regeneration.

中文翻译：

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矿化胶原蛋白对下颌骨再生的BMP-2和VEGF的增强作用。

修复颅面骨骼的损伤具有挑战性。颅面骨需要进行膜内骨化以通过血管生成和成骨作用生成组织工程化的骨移植物。在这里，我们设计了用于BMP-2和血管内皮生长因子（VEGF）的矿化胶原蛋白递送系统，用于植入到下颌骨缺损动物模型中。将BMP-2 / VEGF与矿化胶原蛋白混合，然后将其植入兔下颌骨中。将动物分为（i）没有生长因子的对照；（ii）仅BMP-2；或（iii）BMP-2和VEGF合用。进行CT扫描和组织切片层析和组织学染色以评估骨修复。在BMP-2和BMP-2-VEGF组中，新的骨形成较高，其中血管生成和成骨作用增强。