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Simultaneous Separation and Quantification of Vitamins by Microemulsion Liquid Chromatography.
Journal of Chromatographic Science ( IF 1.5 ) Pub Date : 2020-06-15 , DOI: 10.1093/chromsci/bmaa022
Tongtong Xu 1 , Hongxing Wu 1 , Aini Zheng 1 , Nuojun Yu 1 , Ning Li 1
Affiliation  

Abstract
Microemulsion eluents have been found to have excellent potential uses in high-performance liquid chromatography (HPLC). Here, a novel, environmentally benign and simple method using concentration/flow-rate double-gradient elution using a microemulsion eluent was used to separate water- and fat-soluble vitamins simultaneously and rapidly. Preliminary screening experiments were performed to determine the optimum column type, surfactant concentration, co-surfactant to surfactant ratio, oil, mobile phase pH and microemulsion concentration. The resolution and analysis time were simultaneously optimized using concentration/flow-rate double-gradient elution. The optimized method simultaneously separated water- and fat-soluble vitamins using a Venusil ASB C8 column and a combination of isocratic and linear gradient elution modes using a microemulsion mobile phase (solvent A) consisting of 3.5% (w/w) sodium dodecyl sulfate, 10.5% (w/w) n-butanol, 0.8% (w/w) n-octanol and 85.2% (w/w) water and water (solvent B) at pH 2.50. The optimum detection wavelength was 283 nm. The method was validated and used to analyze a solid pharmaceutical sample.


中文翻译:

通过微乳液相色谱法同时分离和定量维生素。

摘要
已发现微乳液洗脱液在高效液相色谱(HPLC)中具有极好的潜在用途。在这里,使用了一种新型的,环境友好的,简单的方法,该方法使用了使用微乳液洗脱液的浓度/流速双梯度洗脱法,可以同时快速地分离水溶性维生素和脂溶性维生素。进行了初步筛选实验,以确定最佳的色谱柱类型,表面活性剂浓度,助表面活性剂与表面活性剂之比,油,流动相pH和微乳液浓度。使用浓度/流速双梯度洗脱同时优化了分离度和分析时间。丁醇,0.8%(w / w)的辛醇和85.2%(w / w)的水以及pH 2.50的水(溶剂B)。最佳检测波长为283 nm。该方法经过验证,可用于分析固体药物样品。
更新日期:2020-07-24
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