Abstract

Microemulsion eluents have been found to have excellent potential uses in high-performance liquid chromatography (HPLC). Here, a novel, environmentally benign and simple method using concentration/flow-rate double-gradient elution using a microemulsion eluent was used to separate water- and fat-soluble vitamins simultaneously and rapidly. Preliminary screening experiments were performed to determine the optimum column type, surfactant concentration, co-surfactant to surfactant ratio, oil, mobile phase pH and microemulsion concentration. The resolution and analysis time were simultaneously optimized using concentration/flow-rate double-gradient elution. The optimized method simultaneously separated water- and fat-soluble vitamins using a Venusil ASB C8 column and a combination of isocratic and linear gradient elution modes using a microemulsion mobile phase (solvent A) consisting of 3.5% (w/w) sodium dodecyl sulfate, 10.5% (w/w) n-butanol, 0.8% (w/w) n-octanol and 85.2% (w/w) water and water (solvent B) at pH 2.50. The optimum detection wavelength was 283 nm. The method was validated and used to analyze a solid pharmaceutical sample.

中文翻译：

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通过微乳液相色谱法同时分离和定量维生素。

摘要

已发现微乳液洗脱液在高效液相色谱（HPLC）中具有极好的潜在用途。在这里，使用了一种新型的，环境友好的，简单的方法，该方法使用了使用微乳液洗脱液的浓度/流速双梯度洗脱法，可以同时快速地分离水溶性维生素和脂溶性维生素。进行了初步筛选实验，以确定最佳的色谱柱类型，表面活性剂浓度，助表面活性剂与表面活性剂之比，油，流动相pH和微乳液浓度。使用浓度/流速双梯度洗脱同时优化了分离度和分析时间。正丁醇，0.8％（w / w）的正辛醇和85.2％（w / w）的水以及pH 2.50的水（溶剂B）。最佳检测波长为283 nm。该方法经过验证，可用于分析固体药物样品。