Abstract

Mitochondrial impairment is recognised as a prominent feature in kidney diseases. Therefore, we investigated whether the effects of resveratrol, L-carnitine, and apelin in the acute kidney injury model were associated with modulation of mitochondrial quality control (QC) related proteins, intra-renal renin-angiotensin (RAS) activity, adenosine triphosphate (ATP) and Na⁺-K⁺ ATPase gene expression. Rats were randomly assigned to 7 groups: Distilled water injected control group, DMSO injected control group, distilled water injected lipopolysaccharide (LPS) group, DMSO injected LPS group, resveratrol injected LPS group, L-carnitine injected LPS group and apelin 13 injected LPS group. We observed that resveratrol, L-carnitine, and apelin treatments altered mitochondrial (QC) related protein levels (Pink1, Parkin, BNIP-3, Drp1, and PGC1α), decreased intra-renal RAS parameters, increased ATP level and upregulated Na⁺-K⁺ ATPase gene expression in renal tissue. Our results provide new insight into the role of mitochondrial quality control and how different antioxidants exert beneficial effects on acute kidney injury.

摘要

线粒体损伤被认为是肾脏疾病的一个突出特征。因此，我们研究了白藜芦醇、左旋肉碱和apelin在急性肾损伤模型中的作用是否与线粒体质量控制（QC）相关蛋白、肾内肾素-血管紧张素（RAS）活性、三磷酸腺苷的调节有关。 ATP) 和 Na ⁺ -K ⁺ATP酶基因表达。大鼠随机分为7组：蒸馏水注射对照组、DMSO注射对照组、蒸馏水注射脂多糖（LPS）组、DMSO注射LPS组、白藜芦醇注射LPS组、左旋肉碱注射LPS组和apelin 13注射LPS组. 我们观察到白藜芦醇、左旋肉碱和 apelin 治疗改变了线粒体 (QC) 相关蛋白水平（Pink1、Parkin、BNIP-3、Drp1 和 PGC1α），降低了肾内 RAS 参数，增加了 ATP 水平并上调了 Na ⁺ - K ⁺ ATPase 基因在肾组织中的表达。我们的研究结果为线粒体质量控制的作用以及不同的抗氧化剂如何对急性肾损伤发挥有益作用提供了新的见解。