ABSTRACT The C4 plants like maize are at an advantage because they exhibit higher carbon conversion efficiency than C3 during photosynthesis. Using the evaluation of photosynthetic phenotypes and subcellular structure, and high-quality transcriptome analysis for four types of leaves from a bundle sheath defective maize mutant (bsd) and 501 wild line, the key target genes, important transcription factors, and specific pathways were obtained, which may regulate the C-concentrating mechanisms and antioxidant protection of the photosynthetic system, gibberellin signaling, ribosome editing, glycolysis, and chlorophyll biosynthesis. Based on these target genes, a novel network with photosynthetic transformation efficiency with oxidative decarboxylation and ribosome regulation was filtered innovatively by Cytoscape, which adds to our understanding for high-efficiency C-fixation and its genetic improvement in C3 and C4 plants. Graphical abstract

中文翻译：

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使用 RNA-seq 对新型玉米 bsd C4 突变体进行转录组分析

摘要 玉米等 C4 植物具有优势，因为它们在光合作用过程中表现出比 C3 更高的碳转化效率。通过对束鞘缺陷玉米突变体（bsd）和501野生株的四种叶片的光合表型和亚细胞结构的评估以及高质量的转录组分析，获得了关键靶基因、重要转录因子和特定途径，它可能调节光合系统的 C 浓缩机制和抗氧化保护、赤霉素信号传导、核糖体编辑、糖酵解和叶绿素生物合成。基于这些靶基因，Cytoscape创新性地筛选出具有光合转化效率、氧化脱羧和核糖体调控的新型网络，这增加了我们对高效 C 固定及其在 C3 和 C4 植物中的遗传改良的理解。图形概要