Mycobacterium tuberculosis TetR family transcriptional regulator Rv1019 is a negative regulator of the mfd‐mazG operon encoding DNA repair proteins,FEBS Letters

当前位置： X-MOL 学术 › FEBS Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Mycobacterium tuberculosis TetR family transcriptional regulator Rv1019 is a negative regulator of the mfd‐mazG operon encoding DNA repair proteins
FEBS Letters ( IF 3.0 ) Pub Date : 2020-06-30 , DOI: 10.1002/1873-3468.13861
Akhil Raj Pushparajan _{1,

2} , Ranjit Ramachandran _{1,

2} , Jijimole Gopi Reji _{1,

2} , Ramakrishnan Ajay Kumar ₁

Affiliation

Rv1019, a member of an uncharacterized tetracycline resistance regulator family of transcriptional regulators of Mycobacterium tuberculosis H37Rv, was found to be differentially expressed during dormancy and reactivation in vitro. In this study, we show that this protein binds to its own promoter and acts as a negative regulator of its own expression. It forms dimers in vitro which is essential for the DNA binding activity. We also show that Rv1019 and downstream genes Rv1020 (mfd) and Rv1021 (mazG) are cotranscribed. Constitutive expression of Rv1019 in M. smegmatis downregulated MSMEG_5423 (mfd) and MSMEG_5422 (mazG), suggesting that Rv1019 negatively regulates these downstream genes which encode key proteins involved in DNA repair. M. smegmatis expressing Rv1019 was found to be sensitive to DNA‐damaging environments, suggesting its role in regulating the DNA damage response in mycobacterium.

中文翻译：

结核分枝杆菌 TetR 家族转录调节因子 Rv1019 是编码 DNA 修复蛋白的 mfd-mazG 操纵子的负调节因子

Rv1019 是结核分枝杆菌 H37Rv 转录调节因子的未表征四环素抗性调节因子家族的成员，被发现在体外休眠和再激活期间差异表达。在这项研究中，我们表明该蛋白质与其自身的启动子结合并充当其自身表达的负调节器。它在体外形成二聚体，这是 DNA 结合活性所必需的。我们还显示 Rv1019 和下游基因 Rv1020 (mfd) 和 Rv1021 (mazG) 是共转录的。Rv1019 在耻垢分枝杆菌中的组成型表达下调 MSMEG_5423 (mfd) 和 MSMEG_5422 (mazG)，表明 Rv1019 负向调节这些下游基因，这些基因编码参与 DNA 修复的关键蛋白。发现表达 Rv1019 的耻垢分枝杆菌对 DNA 损伤环境敏感，

更新日期：2020-06-30

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11