Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C,STEM CELLS

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Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C
STEM CELLS ( IF 4.0 ) Pub Date : 2020-07-04 , DOI: 10.1002/stem.3243
Hal E Broxmeyer ₁ , Scott Cooper ₁ , Maegan L Capitano ₁

Affiliation

The number of hematopoietic stem cells (HSCs) collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self‐renewing NSG immune‐deficient mouse engrafting and SCID‐repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as noted previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost‐effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.

中文翻译：

在 4°C 下增强表型和移植人脐带血造血干细胞的收集

婴儿出生时在脐带血 (CB) 中收集的造血干细胞 (HSC) 数量是在造血细胞移植 (HCT) 中有效使用 CB 的限制因素。我们现在证明，在婴儿出生后几分钟内在 4°C 下收集和处理人类 CB 会显着增加严格定义的表型 HSC 和自我更新的 NSG 免疫缺陷小鼠移植和 SCID 再生细胞的数量。这与造血祖细胞 (HPC) 的数量减少有关，正如之前针对缺氧收集/处理的细胞阻止环境空气诱导的 HSC 向 HPC 分化所指出的那样。因此，我们定义了一种简单、具有成本效益的方法来收集更多数量的 CB HSC，这些方法可能用于临床 CB HCT。

更新日期：2020-07-04

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