Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes in Rhopalosiphum padi,Insect Science

当前位置： X-MOL 学术 › Insect Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes in Rhopalosiphum padi
Insect Science ( IF 2.9 ) Pub Date : 2020-06-15 , DOI: 10.1111/1744-7917.12842
Xiong Peng ₁ , Cheng Chen ₁ , Yixiao Huang ₁ , Suji Wang ₁ , Sha Su ₁ , Maohua Chen ₁

Affiliation

The short neuropeptide F (sNPF) and NPF receptor (NPFR) genes play important roles in many physiological processes. However, information on the survival-related functions of sNPF and NPFR under different stress conditions is lacking in aphids. In this study, we cloned sNPF and NPFR, and investigated the expression levels of these genes in different developmental stages, wing morphs, and stress conditions of the bird cherry-oat aphid (Rhopalosiphum padi L.), an important agricultural pest. The sNPF and NPFR transcript levels varied among developmental stages, and their expression levels in alate females were significantly higher than those in apterous females. In addition, starvation resulted in significantly increased sNPF expression, which then recovered after refeeding. Heat stress and insecticides significantly affected transcription of both genes. sNPF and NPFR knockdown in R. padi using RNA interference revealed optimal interference efficiency at 48 h post-injection. sNPF knockdown significantly decreased adult longevity, 15-d fecundity, and food intake. Additionally, mortality under starvation, insecticides, and heat stress conditions was significantly higher after injection with double-stranded sNPF in R. padi. NPFR knockdown significantly affected food intake and starvation resistance in R. padi. These results strongly indicate that sNPF plays vital roles in food intake, longevity, and reproduction in R. padi, and it can significantly affect the pest's response to stress conditions.

中文翻译：

Rhopalosiphum padi短神经肽F和NPF受体基因的表达模式及功能分析

短神经肽F ( sNPF ) 和NPF 受体( NPFR ) 基因在许多生理过程中发挥重要作用。然而，在蚜虫中缺乏关于sNPF和NPFR在不同胁迫条件下的生存相关功能的信息。在本研究中，我们克隆了sNPF和NPFR，并研究了这些基因在重要农业害虫鸟樱桃-燕麦蚜虫 ( Rhopalosiphum padi L.)不同发育阶段、翅膀形态和胁迫条件下的表达水平。该sNPF和NPFR转录水平因发育阶段而异，它们在有晚年的雌性中的表达水平显着高于在无性雌性中的表达水平。此外，饥饿导致 s NPF表达显着增加，然后在重新喂食后恢复。热应激和杀虫剂显着影响了这两个基因的转录。在R. padi 中使用 RNA 干扰的sNPF和NPFR敲低显示在注射后 48 小时的最佳干扰效率。sNPF敲低显着降低了成年寿命、15 天的繁殖力和食物摄入量。此外，在饥饿、杀虫剂和热应激条件下的死亡率在注射双链sNPF在R.蚜。NPFR敲低显着影响了R. padi 的食物摄入量和抗饥饿性。这些结果有力地表明sNPF在R. padi 的食物摄入、寿命和繁殖中起着至关重要的作用，并且可以显着影响害虫对胁迫条件的反应。

更新日期：2020-06-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11