Identification of G protein-coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary,Insect Science

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Identification of G protein-coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary
Insect Science ( IF 2.9 ) Pub Date : 2020-06-14 , DOI: 10.1111/1744-7917.12841
Hongyuan Zheng ₁ , Baojuan Zeng ₁ , Tiantian Shang ₁ , Shutang Zhou ₁

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G protein-coupled receptors (GPCRs), a superfamily of integral transmembrane proteins regulate a variety of physiological processes in insects. Juvenile hormone (JH) is known to stimulate Vitellogenin (Vg) synthesis in the fat body, secretion into the hemolymph and uptake by developing oocytes. However, the role of GPCRs in JH-dependent insect vitellogenesis and oocyte maturation remains elusive. In the present study, we performed transcriptomic analysis and RNA interference (RNAi) screening in vitellogenic females of the migratory locust Locusta migratoria. Of 22 GPCRs identified in ovarian transcriptome, LGR4, OR-A1, OR-A2, Mthl1, Mthl5 and Smo were most abundant in the ovary. By comparison, mAChR-C expressed at higher levels in the fat body, whereas Oct/TyrR, OARβ, AdoR and ADGRA3 were at higher expression levels in the brain. Our RNAi screening demonstrated that knockdown of six GPCRs resulted in defective phenotypes of Vg accumulation in developing oocytes, accompanied by blocked ovarian development and impaired oocyte maturation. While LGR4 and Oct/TyrR appeared to control Vg synthesis in the fat body, OR-A1, OR-A2, mAChR-C and CirlL regulated Vg transportation and uptake. The findings provide fundamental evidence for deciphering the regulatory mechanisms of GPCRs in JH-stimulated insect reproduction.

中文翻译：

具有全景卵巢的昆虫卵黄生成和卵发育所需的 G 蛋白偶联受体的鉴定

G 蛋白偶联受体 (GPCRs)，一个跨膜蛋白的超家族，调节昆虫的各种生理过程。已知保幼激素 (JH) 可刺激脂肪体内的卵黄蛋白原 (Vg) 合成、分泌到血淋巴中并被发育中的卵母细胞摄取。然而，GPCR 在 JH 依赖性昆虫卵黄生成和卵母细胞成熟中的作用仍然难以捉摸。在本研究中，我们对飞蝗 Locusta migratoria 的卵黄雌性进行了转录组学分析和 RNA 干扰 (RNAi) 筛选。在卵巢转录组中鉴定的 22 个 GPCR 中，LGR4、OR-A1、OR-A2、Mthl1、Mthl5和Smo在卵巢中含量最高。相比之下，mAChR-C在脂肪体中的表达水平更高，而Oct/TyrR、OARβ、AdoR和ADGRA3在大脑中的表达水平更高。我们的 RNAi 筛选表明，6 个GPCR的敲低导致发育中的卵母细胞中 Vg 积累的表型缺陷，伴随着卵巢发育受阻和卵母细胞成熟受损。虽然 LGR4 和 Oct/TyrR 似乎控制脂肪体内 Vg 的合成，但 OR-A1、OR-A2、mAChR-C 和 CirlL 调节 Vg 的运输和摄取。这些发现为破译 JH 刺激的昆虫繁殖中 GPCR 的调控机制提供了基础证据。

更新日期：2020-06-14

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