Bioassay‐Guided Fractionation, Chemical Compositions and Antibacterial Activity of Extracts from Rhizomes of Globba schomburgkii Hook.f.,Chemistry & Biodiversity

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Bioassay‐Guided Fractionation, Chemical Compositions and Antibacterial Activity of Extracts from Rhizomes of Globba schomburgkii Hook.f.
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-07-28 , DOI: 10.1002/cbdv.202000173
Naruemon Suekaew ₁ , Sarisa Na Pombejra ₂ , Chadin Kulsing ₃ , Jeerapat Doungchawee ₄ , Tanatorn Khotavivattana _{1,

5}

Affiliation

Bioassay-guided fractionation was conducted on dichloromethane extract from the rhizomes of Globba schomburgkii Hook.f. which have previously been reported as the part with the highest antibacterial activity. 10 fractions and 20 sub-fractions were obtained and evaluated for their potency against various strains of bacteria. The most active sub-fractions were 8 times more effective against Staphylococcus aureus and Micrococcus luteus than the original crude extract. Moreover, 2 pure compounds namely petasol and (E)-15,16-dinorlabda-8(17),11-dien-13-one were successfully isolated and characterized, for the first time from this plant species. Untargeted compound analysis of all fractions and sub-fractions was performed by gas chromatography hyphenated with mass spectrometry, leading to positive identification of 167 compounds according to comparison with the mass spectrum and retention index database, 137 of which have never been reported for G. schomburgkii. The correlation between antibacterial activity and composition of each fraction suggests that the bioactive compounds could be 4,8-β-epoxy-caryophyllene, methyl isocostate, (E)-labda-8(17),12-diene-15,16-dial, α-kessyl acetate, zederone, clovanediol, ledene oxide-(I), alantolactone, or 8α,11-elemadiol.

中文翻译：

Globba Schomburgkii Hook.f. 根茎提取物的生物测定引导分馏、化学成分和抗菌活性。

对 Globba schomburgkii Hook.f 根茎的二氯甲烷提取物进行了生物测定引导的分级分离。之前被报道为具有最高抗菌活性的部分。获得了 10 个级分和 20 个亚级分，并评估了它们对各种细菌菌株的效力。最活跃的子级分对金黄色葡萄球菌和藤黄微球菌的有效作用是原始粗提物的 8 倍。此外，首次从该植物物种中成功分离和表征了 2 种纯化合物，即 petasol 和 (E)-15,16-dinorlabda-8(17),11-dien-13-one。通过与质谱联用的气相色谱法对所有馏分和亚馏分进行非目标化合物分析，根据与质谱和保留指数数据库的比较，最终确定了 167 种化合物，其中 137 种从未报道过 G. schomburgkii。抗菌活性与各组分组成之间的相关性表明，生物活性化合物可能是 4,8-β-环氧-石竹烯、异椰子酸甲酯、(E)-labda-8(17),12-diene-15,16-dial , α-kessyl 醋酸盐, zederone, clovanediol, ledene oxide-(I), alantolactone, or 8α,11-elemadiol.

更新日期：2020-07-28

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