当前位置：
X-MOL 学术
›
Biotechnol. Bioeng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Alveolar mimics with periodic strain and its effect on the cell layer formation.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-06-15 , DOI: 10.1002/bit.27458 Milad Radiom 1 , Yong He 2 , Juan Peng-Wang 2 , Armelle Baeza-Squiban 3 , Jean-François Berret 1 , Yong Chen 2
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-06-15 , DOI: 10.1002/bit.27458 Milad Radiom 1 , Yong He 2 , Juan Peng-Wang 2 , Armelle Baeza-Squiban 3 , Jean-François Berret 1 , Yong Chen 2
Affiliation
|
We report on the development of a new model of alveolar air–tissue interface on a chip. The model consists of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40–80 µm at the air–liquid interface by application of air pressure in the range of 200–1,000 Pa. With respect to the diameter of the monolayers, that is, 500 µm, this displacement corresponds to a linear strain of 2–10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time of 1–3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz for 1 hr to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy.
中文翻译：
具有周期性应变的肺泡模拟物及其对细胞层形成的影响。
我们报告了芯片上肺泡空气-组织界面的新模型的开发。该模型由一系列由微框架支撑的悬浮六边形明胶纳米纤维单层和用于贴片集成的微流体装置组成。通过施加 200-1,000 Pa 范围内的气压，悬浮的单层在气-液界面处变形为 40-80 µm 的中心位移。关于单层的直径，即 500 µm，这位移对应于 2-10% 的线性应变，与肺泡中的生理应变范围一致。A549 细胞在单层上培养 1-3 天的培养时间在模型中显示出活力。我们以 0.2 Hz 的频率对细胞施加 5% 的周期性应变，持续 1 小时。我们发现细胞与纳米纤维强烈耦合，但应变降低了耦合并诱导肌动蛋白细胞骨架的重塑，从而导致更好的组织形成。我们的模型可以作为肺部研究的多功能工具，例如吸入毒理学和治疗。
更新日期：2020-08-14
中文翻译：
具有周期性应变的肺泡模拟物及其对细胞层形成的影响。
我们报告了芯片上肺泡空气-组织界面的新模型的开发。该模型由一系列由微框架支撑的悬浮六边形明胶纳米纤维单层和用于贴片集成的微流体装置组成。通过施加 200-1,000 Pa 范围内的气压，悬浮的单层在气-液界面处变形为 40-80 µm 的中心位移。关于单层的直径，即 500 µm，这位移对应于 2-10% 的线性应变，与肺泡中的生理应变范围一致。A549 细胞在单层上培养 1-3 天的培养时间在模型中显示出活力。我们以 0.2 Hz 的频率对细胞施加 5% 的周期性应变，持续 1 小时。我们发现细胞与纳米纤维强烈耦合，但应变降低了耦合并诱导肌动蛋白细胞骨架的重塑，从而导致更好的组织形成。我们的模型可以作为肺部研究的多功能工具，例如吸入毒理学和治疗。
京公网安备 11010802027423号