Abstract The organic acid rich acidogenic liquid produced from waste sludge anaerobic fermentation was used as a potential feedstock for polyhydroxyalkanoates (PHA) production, which is a cost-effective and environmentally sustainable PHA production system. To analyze the effect of substrate on monomer composition of PHA, different acidogenic liquid generated from waste sludge fermentation in varied initial pH were used as external carbon source. The maximal PHA content accounted for 60.3 % of the dry cell with initial fermentation pH 10.0 sludge acidogenic liquid and the recovered polymer composition was 98.3 % polyhydroxybutyrate (PHB) and 1.7 % polyhydroxyvalerate (PHV) by mass. The utilization efficiency of soluble chemical oxygen demand (SCOD), protein, NH4+-N and volatile fatty acids (VFAs) was 95.4 %, 90.5 %, 98.0 %, 99.3 %, respectively. The tyrosine-like protein, tryptophan-like protein, fulvic acid-like organics and soluble microbial by-products were primary used for PHA production, while the humic acid-like organics used little. Furthermore, mass balance analysis was introduced to further illustrate the technology reliability.

中文翻译：

---

以酸或碱预处理污泥产酸液为碳源生产聚羟基链烷酸酯（PHA）：底物代谢与单体组成

摘要 利用废污泥厌氧发酵产生的富含有机酸的产酸液作为聚羟基链烷酸酯（PHA）生产的潜在原料，是一种具有成本效益且环境可持续的PHA生产系统。为了分析底物对PHA单体组成的影响，将废污泥在不同初始pH下发酵产生的不同产酸液用作外部碳源。最大 PHA 含量占干细胞的 60.3%，初始发酵 pH 值为 10.0 污泥产酸液，回收的聚合物组成为 98.3% 聚羟基丁酸酯 (PHB) 和 1.7% 聚羟基戊酸酯 (PHV)。可溶性化学需氧量（SCOD）、蛋白质、NH4+-N和挥发性脂肪酸（VFAs）的利用效率分别为95.4%、90.5%、98.0%、99.3%。酪氨酸样蛋白、色氨酸样蛋白、富里酸样有机物和可溶性微生物副产物主要用于 PHA 生产，而腐植酸样有机物使用很少。此外，引入了质量平衡分析以进一步说明技术可靠性。