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GC–MS ‘chemophenetics’ on Australian pink-flowered Phebalium (Rutaceae) using herbarium leaf material demonstrates phenetic agreement with putative new species
Phytochemistry Letters ( IF 1.3 ) Pub Date : 2020-06-13 , DOI: 10.1016/j.phytol.2020.05.014
Nicholas J. Sadgrove , Ian R.H. Telford , Guillermo F. Padilla-González , Ben W. Greatrex , Jeremy J. Bruhl

The aromatic pink-flowered Phebalium complex is comprised by P. nottii Maiden & Betche, P. speciosum I. Telford and the very similar but mostly white-flowered P. woombye (F.M.Bailey) Domin. Phebalium nottii, as currently circumscribed, includes several geographically disjunct populations that have been segregated at the N.C.W. Beadle Herbarium as putatively distinct species, several of high conservation value. To study volatiles sustainably, large-scale hydrodistillation was aborted and only single leaves were removed from herbarium vouchers, extracted into solvent and analysed by GC–MS. Unlike other species of Phebalium, volatiles in pink-flowered Phebalium are mostly dominated by spathulenol and bicyclogermacrene. Multivariate analysis of the chemistry of volatiles clearly demonstrated good agreement between chemotypes and putative taxa in central Queensland populations. Southern taxa, and the true P. nottii sensu stricto from northern Queensland, express high levels of volatile coumarins. Thus, morphological relationships are mostly reflected in morphometric clusters. As with other members of Phebalium, most taxa can be distinguished on chemistry alone, using only GC–MS analysis of herbarium material. These results corroborate several new species and reveal relationships within the heterogeneous species aggregate of P. nottii.



中文翻译:

使用植物标本室叶材料对澳大利亚粉红色的蕨属植物(芸香科)进行GC-MS的“化学研究”,表明与假定的新物种有物一致

芳香粉红色花Phebalium复合物通过由P. nottii少女&Betche,P. speciosum I.德福和非常相似,但多为白色花P. woombye(FMBailey)DOMIN。目前限定的Phebalium nottii包括几个地理上分离的种群,这些种群在NCW Beadle植物标本室被隔离为公认的物种,其中一些具有很高的保护价值。为了可持续地研究挥发物,中止了大规模加氢蒸馏,只从植物标本室凭证中取出了单片叶子,提取到溶剂中并通过GC-MS分析。不同于其他种类的Phebalium,挥发粉红色的花Phebalium大部分被spathulenol和双环大马来烯占主导。挥发物化学成分的多变量分析清楚地表明,昆士兰州中部人群的化学型与推定的分类单元之间具有良好的一致性。南部的分类单元和昆士兰州北部的真正的P. nottii sensustricto,都表现出高水平的挥发性香豆素。因此,形态学关系主要反映在形态计量学集群中。与Phebalium的其他成员一样,仅使用GC-MS对标本馆材料进行分析,就可以将大多数类群从化学上加以区分。这些结果证实了几种新物种,并揭示了体育杂种异质种聚集体之间的关系。

更新日期:2020-06-13
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