The CO₂ fixation by autotrophic microbes has an important significance for improving carbon sequestration in composting. In this work, the succession of autotrophic CO₂-fixing bacterial communities was investigated using quantitative PCR and high-throughput sequencing of the ribulose-1,5-bisphosphate carboxylase/oxygenase genes (cbbL and cbbM). The results presented that the number of autotrophic CO₂-fixing bacteria was comparable to that in the soil, and most species have not been classified into known bacterial groups, only part of them was assigned into Proteobacteria and Actinobacteria. Phylogenetic analysis displayed that the dominant known cbbL-containing bacteria were Allochromatium vinosum, Rhodobacter sphaeroides, Nitrobacter winogradskyi, Paracoccus yeei and Porphyrobacter sp. CACIAM 03H1, while the dominant known cbbM-containing bacteria were Sulfuritalea hydrogenivorans, Pseudomonas resinovorans, Achromobacter xylosoxidans, Sphingopyxis macrogoltabida and Thermomonospora curvata. In addition, canonical correspondence analysis showed that the evolution of autotrophic CO₂-fixing bacterial communities was greatly affected by physico-chemical parameters such as temperature, C/N and pH.

自养微生物对CO _2的固定对改善堆肥中的固碳具有重要意义。在这项工作中，使用定量PCR和高通量测序核糖-1,5-双磷酸羧化酶/加氧酶基因（cbbL和cbbM）研究了自养性CO ₂固定细菌群落的连续性。结果表明，自养CO ₂固定细菌的数量与土壤中的数量相当，并且大多数物种尚未归类为已知细菌类，只有其中一部分被归类为Proteobacteria和Actinobacteria。系统发育分析表明，已知的显性cbbL含细菌是Allochromatium vinosum，红假单胞菌，硝化winogradskyi，副球菌yeei和Porphyrobacter SP。CACIAM 03H1，而占主导地位的知名CBBM含细菌是Sulfuritalea hydrogenivorans，假单胞菌resinovorans，木糖氧化无色杆菌，鞘脂单胞macrogoltabida和Thermomonospora curvata。此外，规范对应分析表明自养CO ₂的演化固定细菌群落受到诸如温度，C / N和pH的理化参数的极大影响。