Comparative studies of the effects of two commercial enzyme formulations on fiber refining were conducted. Extensive basic characterisation of the enzymes involved, assessment of their hydrolytic activities on different model substrates as well as on different pulps (softwood sulfate, softwood sulfite, hardwood sulfate) were evaluated. Both enzyme formulations showed endoglucanase as well as some xylanase and β-glucosidase activity. In addition, Enzyme A reached a CMC end viscosity of 19.5 mPa compared to 11.1 mPa for Enzyme B. Reducing sugar release almost doubled from 695 μmol mL⁻¹ for hardwood sulfate pulp to 1300 μmol mL⁻¹ for softwood sulfite pulp with Enzyme B under the same conditions. Enzyme A increased the degree of refining even under non-ideal conditions from 23 °SR to up to 50 °SR. Further characterization of hand sheets, made from enzyme pre-treated and refined cellulose fibers with Enzyme A and B, showed that Enzyme A had the best effects leading to hand sheets with increased tensile strength and low air permeability. In summary, the increase in the degree of refining seen for Enzyme A correlated to higher xylanase and β-glucosidase activity and lower endoglucanase activity.

进行了两种商业酶制剂对纤维精制效果的比较研究。评估了所涉及酶的广泛基本特性，评估了它们在不同模型底物上以及在不同纸浆（硫酸软木，亚硫酸软木，硫酸硬木）上的水解活性。两种酶制剂均显示内切葡聚糖酶以及一些木聚糖酶和β-葡萄糖苷酶活性。另外，酶A的CMC最终粘度为19.5 mPa，而酶B的11.1 mPa。还原糖释放量几乎从硬木硫酸盐纸浆的695μmolmL ^-1增加了一倍，达到1300μmolmL ^-1在相同条件下用酶B制成的针叶亚硫酸盐纸浆。即使在非理想的条件下，酶A的提纯程度也从23°SR提高到了50°SR。由酶预处理和精制的纤维素纤维与酶A和B制成的手抄纸的进一步表征表明，酶A的效果最好，导致手抄纸具有更高的拉伸强度和较低的透气性。总之，对于酶A，提纯程度的提高与更高的木聚糖酶和β-葡萄糖苷酶活性以及更低的内切葡聚糖酶活性相关。