This study was performed to investigate whether visible properties of trees species (leaf shape and deciduous property) exhibit strong effects on soil microbial community (SMC). Herein, forest soil and leaves were collected from six pure planted forests of City Forestry Demonstration Base at Harbin, northeast China. These forest stands included Pinus tabuliformis Carr. var. mukdensis Uyeki, P. sylvestris L. var. mongolica Litv, Larix gmelinii (Rupr.) Kuzen, Betula platyphylla Suk, Quercus mongolica Fisch. ex Ledeb, and Fraxinus mandschurica Rupr. The clean soil samples combined with the leaves were incubated in vitro to simulate the degradation of leaves. Analysis of soil fungal microbial community (SFC) and soil bacterial community (SBC) structures by soil fungal internal transcribed spacer (ITS) and bacterial 16S ribosomal RNA gene sequencing resulted that tree species could shape the microbial relative abundance, dominant microbial operational taxonomic unit (OTU), and even SMC structure. Further, analysis on SMCs was performed to test our hypothesis that SFC and SBC structures were correlated with leaf shape (needle or broad) and deciduous property (evergreen or deciduous). Our findings accepted the partial hypothesis that dominant fungal OTUs were sensitive to leaf shape and deciduous property, but could not alter the SFC structure (p > 0.05). A dominant bacterial OTU and not significantly different SBC structure was shared by the leaf shape groups (r = 0.1694, p = 0.0250); while deciduous property determined the dominant bacterial OTU and SBC structure (r = 0.4743, p = 0.0030). The present study provides an insight into the effects of visible properties of tree species on SMC structures.

进行这项研究以调查树木物种的可见特性（叶片形状和落叶特性）是否对土壤微生物群落（SMC）表现出强烈的影响。本文的土壤和树叶采自东北哈尔滨市城市林业示范基地的六个纯种人工林。这些林分包括油松（Pinus tabuliformis Carr）。变种 mukdensis Uyeki，P. sylvestris L. var。蒙古扁豆，落叶松（Rarix gmelinii（Rupr。）Kuzen，白桦（Betula platyphylla Suk），蒙古栎（Quercus mongolica Fisch）。前莱德（Ledeb）和水曲柳（Fraxinus mandschurica Rupr）。将干净的土壤样品与叶子结合在一起进行体外培养模拟叶子的降解。通过土壤真菌内部转录间隔区（ITS）和细菌16S核糖体RNA基因测序分析土壤真菌微生物群落（SFC）和土壤细菌群落（SBC）结构，结果表明树种可以塑造微生物相对丰度，优势微生物操作分类单位（ OTU），甚至SMC结构。此外，对SMC进行分析以检验我们的假设，即SFC和SBC结构与叶片形状（针状或阔叶）和落叶性（常绿或落叶）相关。我们的发现接受了部分假设，即优势真菌OTU对叶片形状和落叶特性敏感，但不能改变SFC结构（p > 0.05）。叶片形状组共有显性细菌OTU，而SBC结构没有显着差异（r = 0.1694，p  = 0.0250 ）；落叶特性决定了细菌的主要OTU和SBC结构（r = 0.4743，p  = 0.0030）。本研究提供了对树种的可见特性对SMC结构的影响的见解。