Background

Alternative glycosylation of serum IgG has been shown to be closely associated with colorectal cancer (CRC). Currently, a dynamic study which can not only minimize the influence of genetic background, environment and other interfering factors during cancer development, but also focus on investigating carcinogenic characteristics of IgG glycan is lacking.

Methods

Serum IgG N-glycans were characterized at four stages of CRC development by ultra-performance liquid chromatography in a typical colitis-related CRC mouse model induced by azoxymethane-dextran sodium sulfate. Furthermore, the expression of related glycosyltransferases in splenic B lymphocytes at the corresponding time was also assessed.

Results

The relative abundance of seven IgG glycans, which can be classified as monoantennary, core fucose, sialic acid, galactose and bisecting, was changed during tumor growth. The abundance of some glycans was altered during the first stage of cancer induction. Correspondingly, the expression of glycosyltransferases in splenic B lymphocytes and different tissues in cancer groups was also decreased compared to that in controls.

Conclusions

This study represents the comprehensive analysis of IgG glycosylation in the dynamic process of colitis-associated CRC. To our knowledge, this is the first report that the expression of glycosyltransferases in mouse splenic B lymphocytes is consistent or inconsistent with the alterations of IgG N-glycans, and the variation tendency is tissue nonspecific.

General Significance

Providing a novel approach to identify the IgG glycans related to the development of CRC and laying a foundation for research on structure and function of glycans using mouse.

中文翻译：

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结肠炎相关结肠癌小鼠模型发展过程中血清IgG N-聚糖谱的动态变化。

背景

血清IgG的替代糖基化已显示与大肠癌（CRC）密切相关。当前，缺乏一种动态研究，其不仅可以最小化遗传背景，环境和其他干扰因素在癌症发展过程中的影响，而且不能集中于研究IgG聚糖的致癌特性。

方法

在由乙氧基甲烷-葡聚糖硫酸钠诱导的典型的结肠炎相关的CRC小鼠模型中，通过超高效液相色谱法在CRC发展的四个阶段对血清IgG N-聚糖进行了表征。此外，还评估了相应时间脾B淋巴细胞中相关糖基转移酶的表达。

结果

在肿瘤生长过程中，七种IgG聚糖的相对丰度发生了变化，可以分为单触角，核心岩藻糖，唾液酸，半乳糖和二等分。在癌症诱导的第一阶段，某些聚糖的丰度发生了变化。相应地，与对照组相比，癌症组的脾脏B淋巴细胞和不同组织中糖基转移酶的表达也降低了。

结论

这项研究代表了结肠炎相关CRC的动态过程中IgG糖基化的全面分析。据我们所知，这是第一个报道，小鼠脾脏B淋巴细胞中糖基转移酶的表达与IgG N-聚糖的变化一致或不一致，并且变化趋势是组织非特异性的。

一般意义

提供了一种新的方法来鉴定与CRC的发展有关的IgG聚糖，并为使用小鼠研究聚糖的结构和功能奠定了基础。