Abstract The two-stage continuously stirred tank reactor (CSTR) is widely used for anaerobic digestion, which shows many advantages over the conventional anaerobic digestion reactor. This work investigated the effects of temperature on the process performance and microbial community structure of two two-stage CSTRs. The volatile solids (VS) removal rate, solubilization percentage and methane (CH4) yield increased from 37.3 ± 5.3 %, 6.2 ± 3.9 % and 74.5 ± 16.8 mL/g VS in the thermophilic-mesophilic anaerobic digestion process (CSTRHi-CSTRM) to 41.4 ± 4.9 %, 19.6 ± 4.2 % and 87.5 ± 20.8 mL/g VS in the thermophilic-thermophilic anaerobic digestion process (CSTRHi-CSTRT), respectively. The effluent volatile fatty acids (VFAs) concentration of CSTRT was higher than that of CSTRM and the accumulation of propionate was observed in CSTRT. The heat of CSTRT to maintain the reactor temperature was 3 times that of CSTRM. The maximum CH4 production rate of CSTRM was 62 % higher than that of CSTRT, and CSTRM had a good methanogenesis potential. The analysis of microbial community structure showed that temperature affected the process performance by changing the species and relative abundance of fermentation bacteria and methanogens.

中文翻译：

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不同温度下污泥厌氧消化的两级连续搅拌釜反应器的工艺性能和微生物相互作用

摘要 两级连续搅拌釜式反应器（CSTR）广泛用于厌氧消化，与传统的厌氧消化反应器相比具有许多优点。这项工作研究了温度对两个两级 CSTR 的工艺性能和微生物群落结构的影响。在高温-中温厌氧消化过程 (CSTRHi-CSTRM) 中，挥发性固体 (VS) 去除率、溶解百分比和甲烷 (CH4) 产率从 37.3 ± 5.3 %、6.2 ± 3.9 % 和 74.5 ± 16.8 mL/g VS 增加到在高温-高温厌氧消化过程 (CSTRHi-CSTRT) 中，VS 分别为 41.4 ± 4.9 %、19.6 ± 4.2 % 和 87.5 ± 20.8 mL/g VS。CSTRT 的出水挥发性脂肪酸 (VFA) 浓度高于 CSTRM，并且在 CSTRT 中观察到丙酸盐的积累。CSTRT 维持反应器温度的热量是 CSTRM 的 3 倍。CSTRM 的最大 CH4 产率比 CSTRT 高 62%，CSTRM 具有良好的产甲烷潜力。微生物群落结构分析表明，温度通过改变发酵细菌和产甲烷菌的种类和相对丰度来影响工艺性能。