Abstract Rumen fluid is used as fresh inoculum for gas production fermentations to predict the nutritional value of feeds and rations for ruminants. However, collection of rumen fluid from animal donors is invasive, expensive, time consuming and results in fluids of variable quality. The general aim was to identify a procedure to manipulate rumen inoculum in order to facilitate its storage and transfer between laboratories. This strategy would also limit fluid collections from animals. Two experiments were completed based on gas production from graduated 100 mL glass syringe with five feeds as substrates. In experiment 1, the gas production and some fermentation parameters of fresh rumen fluids were compared with those preserved at 4 °C for 24, 48, 72 and 96 h. Refrigeration did not modify concentration of volatile fatty acids and pH, but ammonia in liquids refrigerated for 48–96 h was higher (P

中文翻译：

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在发酵 24 小时测量的瘤胃液，冷藏或从冷藏颗粒重组，对气体产量的影响

摘要 瘤胃液用作产气发酵的新鲜接种物，以预测反刍动物饲料和日粮的营养价值。然而，从动物捐献者处收集瘤胃液是侵入性的、昂贵的、耗时的，并且会产生质量参差不齐的液体。总体目标是确定一种操作瘤胃接种物的程序，以促进其在实验室之间的储存和转移。这种策略也将限制从动物身上收集液体。基于从带有五个进料作为底物的带刻度的 100 mL 玻璃注射器产生的气体，完成了两个实验。在实验 1 中，将新鲜瘤胃液的产气量和一些发酵参数与在 4°C 下保存 24、48、72 和 96 小时的那些进行了比较。冷藏不会改变挥发性脂肪酸的浓度和 pH 值，