Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeae-maydis, is one of the most serious foliar diseases of maize worldwide. In the present study, a population of recombinant inbred lines (RILs) derived from a gray leaf spot–resistant inbred line Qi319 and a susceptible line Ye478 was used to map quantitative trait loci (QTL) for GLS resistance. For initial QTL analysis, genetic maps containing 199 simple sequence repeat (SSR) markers were constructed. One major QTL, qGLS1.02, was consistently detected between SSR markers umc2614 and bnlg1803, and explained 6.86–36.24% of the phenotypic variation in GLS resistance. The chromosome segment substitution lines harboring the qGLS1.02 from Qi319 were more resistant to GLS (P < 0.01). Using a recombinant-derived progeny test strategy, the interval containing qGLS1.02 was narrowed to ~ 314 kb according to the B73 (RefGen_v4 genome) including 12 candidate genes; only six of them have been annotated functionally.

由真菌病原菌Cercospora zeae-maydis引起的灰叶斑病（GLS）是全世界玉米最严重的叶面病之一。在本研究中，从抗灰叶斑点的近交系Qi319和易感系Ye478衍生的重组近交系（RIL）群体用于绘制GLS抗性的定量性状基因座（QTL）。对于初始QTL分析，构建了包含199个简单序列重复（SSR）标记的遗传图谱。在SSR标记umc2614和bnlg1803之间一致地检测到一个主要的QTL qGLS1.02，解释了GLS抗性的表型变异的6.86–36.24％。携带Qi319 qGLS1.02的染色体片段替代系对GLS的抗性更高（P <0.01）。使用重组衍生的后代测试策略，根据包含12个候选基因的B73（RefGen_v4基因组），将包含qGLS1.02的间隔缩小到〜314 kb；其中只有六个功能上有注释。