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Evidence of genetic diversity within Solanum Lycopersicum L. ‘Platense’ landrace and identification of various subpopulations
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2020-06-14 , DOI: 10.1007/s10722-020-00959-8
Raúl Amado Cattáneo , Andrés Norman McCarthy , Sergio Enrique Feingold

Local varieties and landraces are traditional crops that have evolved over time through adaptation to their natural and cultural environment. They are presently regarded as a valuable genetic reservoir, given that most of the genetic diversity of domesticated species lies in these and other traditionally used varieties. The 'Platense' tomato landrace is adapted to the local soil and climate of the La Plata horticultural green belt, as a result of the gradual selection of a number of tomato cultivars that were introduced in Argentina towards the end of the nineteenth century following the massive immigration flow, mainly from Europe. In the present study we have evaluated the genetic identity and diversity of this landrace and all its subpopulations registered at the Germplasm Bank of the “La Consulta” Agricultural Experimental Station of INTA. The molecular analysis, based on 14 polymorphic microsatellite markers, presented a mean number of alleles per locus of 2.56 with an average polymorphic information content of 0.28. The genetic information obtained allowed the assessment of the genetic variation between and within entries, as well as the identification of all revealed genotypes with an exclusion power of over 99.99%. Likewise, the diversity was evaluated for the whole landrace, as well as for every subpopulation, establishing notably high diversity values both for the complete landrace and a number of its subpopulations. This information is highly useful in the description of the 'Platense' landrace variability and constitutes a solid approach for the genetic characterization of the available material at Germplasm Bank accessions, as well as for other eventual members of this landrace presently in use by local horticulturists.



中文翻译:

茄子“ Platense”地方品种内遗传多样性的证据以及各种亚群的鉴定

当地品种和地方品种是传统农作物,通过适应其自然和文化环境而逐渐发展。鉴于驯化物种的大多数遗传多样性都存在于这些和其他传统使用的品种中,因此它们目前被视为宝贵的遗传资源。“ Platense”番茄地方品种适应了La Plata园艺绿化带的当地土壤和气候,这是由于在19世纪末大规模引进番茄品种之后,阿根廷逐渐选择了一些番茄品种移民流主要来自欧洲。在本研究中,我们评估了该地方品种及其所有亚种群在INTA“ La Consulta”农业实验站种质库中注册的遗传特性和多样性。分子分析基于14个多态性微卫星标记,得出每个位点的平均等位基因数为2.56,平均多态性信息含量为0.28。获得的遗传信息可以评估条目之间和条目之内的遗传变异,并鉴定出所有显示的基因型,排他力超过99.99%。同样,对整个地方品种以及每个子种群的多样性进行了评估,从而为整个地方品种及其许多子种群建立了显着的高多样性值。

更新日期:2020-06-14
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