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Identification of Citruses from Montenegro Based on Microsatellite Clustering Analyses
Erwerbs-Obstbau ( IF 1.3 ) Pub Date : 2020-06-13 , DOI: 10.1007/s10341-020-00508-8
Lidija Bitz , Slavojka Malidzan , Natasa Stajner , Teija Tenhola-Roininen , Branka Javornik

A set of 32 citrus genotypes were selected from Montenegro collection and analyzed by the use of 11 microsatellite markers. Analyses harvested 72 different alleles having 6.5 alleles per locus and discovered presence of 23 unique genotypes. Distance analysis and model-based clustering revealed both the presence of 4 groups: satsuma (Citrus unshiu Marc.) mandarins, clementine (Citrus reticulata) mandarins, sweet oranges (Citrus × sinensis) and lemons (Citrus limon). Distance based clustering enabled the differentiation between red and yellow flashed fruits due to the presence of allele 158 on the locus AC01 in blood oranges. Specific goal of the study was to clarify the identity of 3 local citrus genotypes through observing their relationships within well-established clustering with the rest of genotypes being known citruses. Local orange ‘Domaca Pomorandza’ grouped with other sweet oranges but could not be differentiated from ‘Navelina’ and ‘Skagss Bonanza Navel’. ‘Gorka Pomorandza’ had unique fingerprint and showed to be sour orange. Local lemon ‘Mjesecar’ showed to may be belonging to the Femminello group of lemons. Genetic characterization of local genotypes will serve for cultivar recognition and their future improvement.

中文翻译:

基于微卫星聚类分析的黑山柑桔鉴定

从黑山收集物中选择了一组32种柑橘基因型,并通过使用11种微卫星标记进行了分析。分析收集了每个位点具有6.5个等位基因的72个不同等位基因,并发现了23种独特的基因型。距离分析和基于模型的聚类揭示了这两个组的存在:萨摩(Cutrus unshiu Marc。)普通话,柑桔(Citrus reticulata)普通话,甜橙(Citrus  ×  sinensis)和柠檬(Citrus limon))。基于距离的聚类能够区分红色和黄色闪烁的水果,这是由于血橙中AC01位点上存在等位基因158。该研究的具体目标是通过观察3种本地柑橘基因型在公认的聚类中与其他已知的柑橘基因型之间的关系来阐明其身份。本地橙“ Domaca Pomorandza”与其他甜橙组合在一起,但无法与“ Navelina”和“ Skagss Bonanza Navel”区分开。“ Gorka Pomorandza”具有独特的指纹,并显示为酸橙。本地柠檬“ Mjesecar”显示可能属于Femminello组柠檬。本地基因型的遗传表征将有助于品种识别及其未来的改良。
更新日期:2020-06-13
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