To explore the effects of aquaporin (AQP) 1 on pregnancy outcome and the association between expression of AQP1 and other AQPs in the placenta and foetal membranes, the rate of copulatory plugs and pregnancy, amniotic fluid (AF) volume, osmolality and composition were determined in AQP1-knockout (AQP1−/−) mice at different gestational days (GD). The expression and location of AQP1 and other AQPs in the placenta and foetal membranes of AQP1−/− mice, AQP1-siRNA transfected WISH cells and oligohydramnios patients were also detected. Compared to control mice, AQP1−/− mice exhibited reduced copulation plug and successful pregnancy rates, but these effects were accompanied by a larger AF volume and lower AF osmolality at late gestation. AQP9 expression was significantly decreased in the placenta and foetal membranes of AQP1−/− mice, while AQP8 level was elevated in the foetal membranes of AQP1−/− mice. Moreover, AQP9 expression was suppressed in WISH cells after AQP1 downregulation. Furthermore, AQP9 expression was associated with AQP1 level in the placenta and foetal membranes in oligohydramnios. AQP1 may play a critical role in regulating pregnancy outcome and maternal-foetal fluid homeostasis. Changes in AQP1 expression may lead to compensatory alterations in AQP8 and AQP9 expression in the placenta.

中文翻译：

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水通道蛋白 1 影响妊娠结局并调节胎盘中水通道蛋白 8 和 9 的表达

为了探讨水通道蛋白 (AQP) 1 对妊娠结局的影响以及 AQP1 与胎盘和胎膜中其他 AQP 表达之间的关系，确定了交配栓率和妊娠、羊水 (AF) 体积、渗透压和成分在不同孕期 (GD) 的 AQP1 基因敲除 (AQP1-/-) 小鼠中。还检测了 AQP1 和其他 AQP 在 AQP1-/- 小鼠、AQP1-siRNA 转染的 WISH 细胞和羊水过少患者的胎盘和胎膜中的表达和定位。与对照小鼠相比，AQP1-/- 小鼠的交配栓和成功妊娠率降低，但这些影响伴随着更大的 AF 体积和较低的妊娠晚期 AF 渗透压。AQP1-/-小鼠胎盘和胎膜中AQP9的表达显着降低，而 AQP1-/- 小鼠胎膜中的 AQP8 水平升高。此外，AQP1 下调后 WISH 细胞中的 AQP9 表达受到抑制。此外，AQP9 表达与羊水过少中胎盘和胎膜中的 AQP1 水平相关。AQP1 可能在调节妊娠结局和母胎体液稳态方面发挥关键作用。AQP1 表达的变化可能导致胎盘中 AQP8 和 AQP9 表达的代偿性改变。