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A study to evaluate the need for comminution in the preparation of food materials for testing of Listeria monocytogenes, Staphylococcus species and generic Escherichia coli
Journal of AOAC INTERNATIONAL ( IF 1.7 ) Pub Date : 2020-06-13 , DOI: 10.1093/jaoacint/qsaa074
Patricia Hanson 1 , Nicole Mitchell 2 , S Brian Caudle 1 , Lyndsey Caulkins 1 , Cameron Owens 1 , Patty Lewandowski 3 , Diane Pickett 1
Affiliation  

Comminution reduces the sampling error arising from the compositional heterogeneity and mixing of the sample during comminution reduces the sampling error arising from distributional heterogeneity of the target contaminant/target analyte in the material, facilitating the selection of a more representative test portion. A laboratory sampling method incorporating comminution prior to selection of the test portion (Sampling Method B) was compared to current sampling methods that used no comminution step (Sampling Method A). This required developing an efficient process for comminution of food samples prior to removal of the test portion for the detection and isolation of Listeria monocytogenes and the enumeration of Staphylococcus species and Escherichia coli. From December 2016 to December 2017, 2,742 tests were conducted on 778 unique food samples. For all food samples, a test portion (TPA) was first removed using a non-comminution method Sampling Method A, and then the remainder of the material was comminuted and a second test portion (TPB) was removed using Sampling Method B and tested along-side the first portion. Across all food matrices and microbial targets, the number of an additional 17 targets were detected using only sampling Method B, an additional positive detections of target analytes increased 77% using Sampling Method B over the number of from the 22 detections from the test portions taken using Sampling Method A.

中文翻译:

评估在粉碎用于测试单核细胞增生李斯特菌葡萄球菌和一般大肠杆菌的食品原料中需要粉碎的研究

粉碎减少了由于成分异质性引起的采样误差,粉碎过程中样品的混合减少了由于目标污染物/目标分析物在材料中的分布不均性引起的采样误差,从而有助于选择更具代表性的测试部分。比较了在选择测试部分之前采用粉碎的实验室采样方法(采样方法B)与不使用粉碎步骤的当前采样方法(采样方法A)。这就要求在去除用于检测和分离单核细胞增生性李斯特氏菌以及枚举葡萄球菌和大肠杆菌的测试部分之前,开发一种有效的食品样品粉碎方法。从2016年12月到2017年12月,对778个独特的食物样本进行了2,742次测试。对于所有食品样品,首先使用非粉碎方法采样方法A去除测试部分(TPA),然后将剩余的材料粉碎,然后使用采样方法B去除第二测试部分(TPB)并进行测试-在第一部分。在所有食品基质和微生物目标中,仅使用采样方法B即可检测到另外17个目标的数量,而使用采样方法B所检测到的目标分析物的其他阳性检测数则比从测试部分获得的22种检测数增加了77%使用抽样方法A。然后将剩余的材料粉碎,并使用采样方法B去除第二测试部分(TPB),并沿着第一部分进行测试。在所有食品基质和微生物目标中,仅使用采样方法B即可检测到另外17个目标的数量,而使用采样方法B所检测到的目标分析物的其他阳性检测数则比从测试部分获得的22种检测数增加了77%使用抽样方法A。然后将剩余的材料粉碎,并使用采样方法B去除第二测试部分(TPB),并沿着第一部分进行测试。在所有食品基质和微生物目标中,仅使用采样方法B即可检测到另外17个目标的数量,而使用采样方法B所检测到的目标分析物的其他阳性检测数则比从测试部分获得的22种检测数增加了77%使用抽样方法A。
更新日期:2020-06-13
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