Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages,ACS Applied Bio Materials

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Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-13 , DOI: 10.1021/acsabm.0c00345
Youngmin Jo ₁ , Hyo Min Kim ₁ , Jongbeom Lee ₁ , Chungha Lee _{2,

3} , Hervé Hugonnet _{2,

3} , YongKeun Park _{2,

3,

4} , Xiao Liu ₅ , Young-Tae Chang ₅ , Hyoungsoo Kim ₆ , Pilnam Kim _{1,

3}

Affiliation

Spontaneous activation of macrophages in response to inflammation is a key part of innate immunity and host defense. Macrophages represent a heterogeneous population of cells with different phenotypic profiles performing distinct functions in host defense. Although a spectrum of macrophage activation stages exists in an inflamed region, the effect of local physical conditions on the heterotypic activation of macrophages is unknown. Here, we introduce an in vivo fluid–matrix interface analogous culture platform, an asymmetric microenvironment, facilitating the formation of macrophage aggregates (MAs). Macrophages were self-assembled to form MAs of ∼100 μm diameter at the collagen matrix–medium interface upon phorbol-12-myristate-13-acetate treatment. The macrophages within the half-embedded MAs into the matrix were heterogeneously activated, resulting in inhomogeneous cell–cell and cell–matrix interactions within the aggregates. Our demonstration may aid in a better understanding of the acquisition of macrophage heterogeneity in response to tissue-specific microenvironments.

中文翻译：

流体-基质界面触发巨噬细胞的异质激活

响应炎症的巨噬细胞自发激活是先天免疫和宿主防御的关键部分。巨噬细胞代表具有不同表型特征的异质细胞群，在宿主防御中发挥不同的功能。尽管炎症区域存在一系列巨噬细胞活化阶段，但局部物理条件对巨噬细胞异型活化的影响尚不清楚。在这里，我们介绍了一种体内流体-基质界面类似培养平台，一种不对称的微环境，促进巨噬细胞聚集体 (MAs) 的形成。在 phorbol-12-myristate-13-acetate 处理后，巨噬细胞在胶原基质 - 培养基界面处自组装形成直径约 100 μm 的 MA。半嵌入基质中的 MA 内的巨噬细胞被异质激活，导致聚集体内的细胞 - 细胞和细胞 - 基质相互作用不均匀。我们的演示可能有助于更好地理解巨噬细胞异质性对组织特异性微环境的反应。

更新日期：2020-07-20

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