Lipids, proteins, and carbohydrates are the major constituents found in microalga cells, in varying proportions, and these biomolecules find applications in different industries. During microalga cultivation, to efficiently manipulate, control, and optimize the productivity of a specific compound for a specific application, real‐time monitoring of these three cell components is essential. In this study, a method using measurement of electrical capacitance was developed to simultaneously determine the lipid, protein, and carbohydrate content of microalga cells without the requirement for any pre‐processing steps. The marine microalga Nannochloropsis oculata was cultivated under nitrogen starvation conditions to induce lipid accumulation over a period of 22 days. The correlation between the electrical capacitance of the microalga culture and the intracellular biomolecule content (determined by standard techniques) was investigated, enabling subsequent deduction of microalga intracellular content from electrical capacitance of the culture. The accuracy and precision of the technique were proven by validating an independent sample. The main advantage of the proposed technique is its capability of quantifying microalga composition within a few minutes, significantly faster than currently available conventional techniques.

中文翻译：

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使用电化学阻抗谱同时快速定量微藻生物分子含量。

脂质、蛋白质和碳水化合物是微藻细胞中发现的主要成分，比例各不相同，这些生物分子在不同的行业中都有应用。在微藻培养过程中，为了针对特定应用有效操纵、控制和优化特定化合物的生产力，对这三种细胞成分的实时监测至关重要。在这项研究中，开发了一种使用电容测量的方法来同时测定微藻细胞的脂质、蛋白质和碳水化合物含量，而无需任何预处理步骤。海洋微藻Nannochloropsis oculata在氮饥饿条件下培养以诱导脂质积累超过 22 天。研究了微藻培养物的电容与细胞内生物分子含量（通过标准技术确定）之间的相关性，从而能够从培养物的电容中推导出微藻细胞内含量。该技术的准确性和精密度通过验证独立样本得到证明。所提出的技术的主要优点是它能够在几分钟内量化微藻成分，比目前可用的传统技术快得多。