Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS‐scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 μM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm–zona pellucida binding capacity were observed in the 50 μM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 μM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility.

中文翻译：

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三七皂苷R1在17°C的液体储存过程中可保护公猪精子。

活性氧（ROS）在液体储存过程中会损害哺乳动物的精子。三七皂苷R1（NR1）是从三七的根中分离出来的化合物; 它具有强大的ROS清除活动。这项工作假设NR1的抗氧化能力可以改善液体储存过程中的公猪精子质量和繁殖力。在17°C的液体储存过程中，保存5天后，向精液补充剂中补充NR1（50μM）可显着改善精子活力，膜完整性和顶体完整性。NR1治疗还降低了第5天的ROS和脂质过氧化（LPO）水平（p <0.05）。在第7天，与对照组相比，在50μMNR1组中观察到更高的谷胱甘肽（GSH），超氧化物歧化酶（SOD），过氧化氢酶（CAT）和精子-透明带结合能力（p  <0.05）。重要的是，对200头母猪的生育能力进行的统计分析表明，向增量剂中添加NR1可以改善17°C储液期间公猪精子的生育能力参数（p  <0.05）。这些结果证明了在17°C的液体储存过程中在公猪补充剂中使用50μMNR1作为抗氧化剂的实际可行性，这有利于精子的质量和生育能力。