Due to the digestible refractory and absorbable structures of bioactive peptides (BPs), they could induce notable biological impacts on the living organism. In this regard, the current study was devoted to providing an overview regarding the available methods for BPs generation by the aid of a systematic review conducted on the published articles up to April 2019. In this context, the PubMed and Scopus databases were screened to retrieve the related publications. According to the results, although the characterization of BPs mainly has been performed using enzymatic and microbial in‐vitro methods, they cannot be considered as suitable techniques for further stimulation of digestion in the gastrointestinal tract. Therefore, new approaches for both in‐vivo and in‐silico methods for BPs identification should be developed to overcome the obstacles that belonged to the current methods. The purpose of this review was to compile the recent analytical methods applied for studying various aspects of food‐derived biopeptides, and emphasizing generation at in vitro, in vivo, and in silico.

中文翻译：

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生物活性肽生成的技术、观点和挑战：综合系统评价

由于生物活性肽（BPs）的可消化难熔和可吸收结构，它们可以对生物体产生显着的生物学影响。在这方面，本研究致力于通过对截至 2019 年 4 月的已发表文章进行系统审查，提供有关生成 BP 的可用方法的概述。在这种情况下，筛选了 PubMed 和 Scopus 数据库以检索相关出版物。根据结果​​，虽然 BPs 的表征主要是使用酶法和微生物体外方法进行的，但它们不能被认为是进一步刺激胃肠道消化的合适技术。因此，体内和硅片的新方法应该开发用于BPs识别的方法来克服属于当前方法的障碍。本综述的目的是汇编最近用于研究食品衍生生物肽各个方面的分析方法，并强调体外、体内和计算机中的生成。