Experimental and model‐based studies of acrylonitrile biohydration catalyzed by free cells were conducted in this work. The high ratios of the two phases of the reaction system and the effects of inhibition and inactivation on the enzyme make the process complicated; thus, its exact kinetics have never been reported. To fully understand and model the reaction, a microstructured chemical system was employed to investigate droplet dispersion, the mass transfer process, the intrinsic kinetics with inhibition and inactivation, and the preparation process of acrylamide. By adjusting the structure size and flow conditions of the microreactor, acrylonitrile droplets of 40 μm were obtained and enhanced mass transfer was achieved. Compared to previous studies, the microchemical system in our work provides more accurate kinetic parameters, with a much higher maximum reaction rate and much smaller Michaelis constant. The numerical predictions of the process model show good agreement with experimental results.

中文翻译：

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在微结构化学系统中基于实验和基于模型的丙烯腈生物水合为丙烯酰胺的研究

在这项工作中，进行了由游离细胞催化的丙烯腈生物水合的实验和基于模型的研究。反应体系两相的比例很高，以及酶的抑制和失活作用使该过程变得复杂。因此，尚未报道其确切的动力学。为了充分理解和模拟反应，采用了微结构化学系统研究液滴的分散，传质过程，具有抑制和失活作用的内在动力学以及丙烯酰胺的制备过程。通过调节微反应器的结构尺寸和流动条件，获得了40μm的丙烯腈液滴，并实现了更高的传质。与以前的研究相比，我们工作中的微化学系统提供了更准确的动力学参数，具有更高的最大反应速率和更小的米氏常数。过程模型的数值预测与实验结果吻合良好。