Soil organic carbon (SOC) derived from manure increase phosphorus (P) availability by increasing the proportion of organic P (Po) in total P. However, what role SOC plays in the process of converting Po to available P (AP) and who modulates Po mineralization are still poorly understood. In this study, we collected three soil samples under long-term filed treatment with different organic (no carbon, straw, and manure) inputs. By comparing bacterial and fungal lecithin-enrichment liquid cultures, we observed that the lecithin-enriched fungal community showed higher capability for Po mineralization. Using high-throughput sequencing of the field and lecithin-enriched microbial communities, we identified Po-mineralizing taxa in the soil. Co-occurrence network analysis revealed that the keystone fungal taxa Geastrum sp. and Chaetomium sp. in the fungal network negatively associated with Po-mineralizing fungal taxa, whereas keystone bacterial taxon was not directly related to Po-mineralizing bacteria. We found labile C limits the growth of keystone fungal taxa and that the addition of lactose enhanced Po mineralization by increasing the abundance of Po-mineralizing fungal taxa. Our results emphasize the importance of soil fungi for Po mineralization in acidic soil from a community perspective and provide evidence that easily degradable C drives Po mineralization and influences P availability through limitation of keystone fungal taxa. Our study gives insight into the biological mechanisms underlying specific organic carbon-induced interactions between fungal taxa and provides crucial information for the facilitation of P cycling.

粪便中的土壤有机碳（SOC）通过增加有机磷（Po）在总磷中的比例来提高磷（P）的利用率。但是，SOC在将Po转化为可用磷（AP）的过程中起什么作用，以及谁进行调节宝矿化仍知之甚少。在这项研究中，我们收集了经过长期处理的三种土壤样品，它们具有不同的有机（无碳，秸秆和肥料）输入。通过比较细菌和真菌富含卵磷脂的液体培养物，我们观察到富含卵磷脂的真菌群落显示出更高的Po矿化能力。使用该领域和卵磷脂丰富的微生物群落的高通量测序，我们确定了土壤中的Po矿化类群。共现网络分析表明，梯形真菌类群Geastrumsp。和Chaetomiumsp。在真菌网络中，与Po矿化真菌类群负相关，而Keystone细菌分类群与Po矿化细菌没有直接关系。我们发现不稳定的C限制了梯形真菌类群的生长，并且乳糖的添加通过增加Po矿化真菌类群的丰度提高了Po的矿化度。我们的研究结果从社区的角度强调了土壤真菌对于酸性土壤中Po矿化的重要性，并提供证据表明易于降解的C驱动Po矿化并通过限制梯形真菌类群影响P的有效性。我们的研究提供了深入了解真菌分类群之间特定有机碳诱导的相互作用的生物学机制，并为促进磷循环提供了重要信息。