An eco-friendly and inexpensive ion-exchange membrane was developed for efficient purification of lysozyme from chicken egg white. Agricultural waste particles (rice husk ash, RHA) were incorporated into biodegradable polymeric matrix (polylatic acid, PLA) to fabricate porous PLA/RHA mixed matrix membrane (MMM) via water-vapour-induced phase inversion. The resulted membrane had an ion-exchange capacity (IEC) of 844 μmol/g membrane. In batch operation at pH 8, the protein adsorption equilibrium was attained within 2 h of contact time and the maximum lysozyme adsorption capacity was 4.49 μmol/g membrane. The adsorptivity of PLA/RHA MMM was essentially contributed from the filled RHA particles. In dynamic operation using one piece of PLA/RHA MMM at 122 L/h/m² for a feed lysozyme concentration identical to that in mucin-free egg white (0.5 mg/mL), the breakthrough volume was about 50 MMM volume. The lysozyme recovery was 68–70% with a near 100% purity and a purification fold of 35 after an adsorption/washing/elution cycle treating mucin-free egg white mixture. The MMM performance was only deteriorated at a minor percentage during three successive cycles. These results demonstrate that the PLA/RHA MMM process is a green and efficient approach for isolating lysozyme from chicken egg white.

为了从鸡蛋白中高效纯化溶菌酶，开发了一种环保且廉价的离子交换膜。将农业废物颗粒（稻壳灰，RHA）掺入可生物降解的聚合物基质（聚乳酸，PLA）中，以通过水蒸气诱导的相转化来制造多孔的PLA / RHA混合基质膜（MMM）。所得膜的离子交换容量（IEC）为844μmol/ g膜。在pH为8的分批操作中，在接触时间内2小时内达到蛋白质吸附平衡，最大溶菌酶吸附容量为4.49μmol/ g膜。PLA / RHA MMM的吸附性主要来自填充的RHA颗粒。在动态运行中使用一块122 L / h / m ²的PLA / RHA MMM对于与不含粘蛋白的蛋清（0.5 mg / mL）相同的饲料溶菌酶浓度，穿透体积约为50 MMM体积。吸附/洗涤/洗脱循环处理无粘蛋白的蛋清混合物后，溶菌酶的回收率为68-70％，纯度接近100％，纯化倍数为35。在三个连续的周期中，MMM性能仅在很小的程度上下降。这些结果表明，PLA / RHA MMM工艺是一种从鸡蛋白中分离溶菌酶的绿色高效方法。