A novel regeneration system through micrografting is reported for the endangered Argania spinosa (L.) Skeels. Rootstocks were obtained from in vitro germinated seeds of the argan genotype G27. It was found that the storage time significantly affects the seed germination capacity, and that the seeds cultured immediately after harvest exhibit the highest germination percentage (91.6%). Besides, transferring seedlings to half-strength Murashige and Skoog (½MS) medium supplemented with 1 mg l⁻¹ gibberellic acid (GA₃) and 1 mg l⁻¹ 6-benzylaminopurine (BAP) resulted in the highest shoot and root lengths (2.05 and 5.73 cm, respectively). Scions were taken from axillary shoots developed in vitro from microcuttings of genotype G41. Micrografting was performed by using the wedge technique. Afterwards, the micrografted plants were transferred to media supplemented with different plant growth regulators (PGRs). After 2 months of culture, 65–100% of the micrografted plants survived, and no difficulties were observed during the formation of the rootstock-scion union. Interestingly, the use of GA₃ at concentrations ranging from 0.1 to 1 mg l⁻¹ was essential for successful micrografting (85–100%) and subsequent growth and development of shoots (2.40–2.72 cm length). After micrografting, scions produced one, two or multiple shoots, depending on PGRs. Histological analysis clearly demonstrated the successful union between rootstocks and scions, with active cell division and vascular tissue formation in the grafting region. After transferring the micrografted plants to the glasshouse, a survival rate of 80% was observed, and the plants showed normal growth and development.

据报道，濒危的Argania spinosa（L.）龙骨通过微移植获得了一种新型的再生系统。从摩洛哥坚果基因型G27的体外发芽种子中获得砧木。发现贮藏时间显着影响种子的发芽能力，并且收获后立即培养的种子表现出最高的发芽率（91.6％）。此外，将幼苗转移到补充有1 mg l ^-1赤霉素（GA ₃）和1 mg l ^-1的半强度Murashige和Skoog（1 / 2MS）培养基中6-苄基氨基嘌呤（BAP）的芽和根长最高（分别为2.05和5.73 cm）。接穗是从基因型G41的微切屑体外发育而来的腋生芽中提取的。通过使用楔形技术进行微移植。之后，将微移植的植物转移到补充了不同植物生长调节剂（PGR）的培养基中。培养2个月后，有65-100％的微嫁接植物得以存活，在砧木-接穗结合的形成过程中没有发现任何困难。有趣的是，GA ₃的使用浓度范围为0.1到1 mg l ^-1对于成功的微移植（85–100％）以及随后的芽生长和发育（2.40–2.72 cm长）至关重要。微移植后，根据PGRs，接穗产生一个，两个或多个芽。组织学分析清楚地证明了砧木和接穗之间的成功结合，在嫁接区有活跃的细胞分裂和血管组织形成。将微移植的植物转移到温室后，观察到存活率为80％，并且植物显示出正常的生长和发育。