In Mexico little is known about high-altitude glacial psychrotolerant or psychrophilic fungal species, with most glacial fungi isolated from polar environments or Alpine glaciers. It has been documented that some of these species may play an important role in bioremediation of contaminated environments with heavy metals. In the present study, 75 fungi were isolated from glaciers in Citlaltépetl (5675 masl) and Iztaccíhuatl (5286 masl) volcanoes. Combining morphological characteristics and molecular methods, based on ITS rDNA, 38 fungi were partially identified to genus level, 35 belonging to Ascomycota and three to Mucoromycota. The most abundant genera were Cladosporium, followed by Alternaria and Sordariomycetes order. All isolated fungi were psychrotolerant, pigmented and resistant to different concentrations of Cr(III) and Pb(II), while none tolerated Hg(II). Fungi most tolerant to Cr(III) and Pb(II) belong to the genera Stemphylium, Cladosporium and Penicillium and to a lesser extent Aureobasidium and Sordariomycetes. To our knowledge, this is the first report on cultivable mycobiota richness and their Cr and Pb tolerance. The results open new research possibilities about fungal diversity and heavy metals myco-remediation. Extremophilic fungal communities should be further investigated before global warming causes permanent changes and we miss the opportunity to describe these sites in Mexico.

中文翻译：

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从西塔尔特佩特尔火山和伊斯塔奇瓦伊特尔火山（墨西哥）的三个高海拔冰川中回收的可培养真菌的丰富度和金属耐受性。

在墨西哥，人们对高海拔冰川抗精神病或嗜酸性真菌物种知之甚少，其中大多数冰川真菌均来自极地环境或高山冰川。据记录，其中一些物种可能在重金属污染环境的生物修复中发挥重要作用。在本研究中，从Citlaltépetl（5675 masl）和Iztaccíhuatl（5286 masl）火山的冰川中分离出75种真菌。结合形态学特征和分子方法，基于ITS rDNA，部分鉴定了38个真菌属，其中35个属于子囊菌，三个为毛霉菌。最丰富的属是枝孢菌属，其次是链格孢属和拟南芥订购。所有分离出的真菌都具有抗精神病能力，色素沉着并且对不同浓度的Cr（III）和Pb（II）具有抵抗力，而Hg（II）则不耐受。对Cr（III）和Pb（II）最耐受的真菌属于Stemphylium，Cladosporium和Penicillium属，较小的是Aureobasidium和Sordariomycetes。据我们所知，这是关于可培养的分枝杆菌丰富度及其对Cr和Pb耐受性的第一份报告。结果为真菌多样性和重金属真菌修复提供了新的研究可能性。在全球变暖造成永久性变化之前，应进一步调查极端微生物的真菌群落，我们错过了描述墨西哥这些遗址的机会。