Abstract

Fish protein hydrolysate, which is released to the environment as tuna processing waste during the hydrolysis reaction of Sarda Orientalis’ black muscle, could be used as a nutritional source of nitrogen for food products because of its valuable properties in antioxidant effects. This study used Protamex protease (endopeptidase) to hydrolyze the tuna’s black muscle (TBM) and to produce fish protein hydrolysate (FPH) for producing nutritional food powder. To access the FPH production, optimized conditions of reaction in hydrolyzing TBM were determined by factorial experimental design with the Box–Behnken model at a ratio of 0.45% enzyme/substrate, 100% added water content and temperature of 56 °C for 4.0 h. Consequently, the FPH obtained with the optimized condition resulted in an average degree of hydrolysis of 7.72% in response to the required range of 5–20%. Moreover, this FPH was detected for compositions of amino acids and peptides with low molecular weight less than 14 kDa and was tested in high antioxidant activity of 70% at its concentration of 500 µg mL⁻¹. This study has provided an efficient procedure to solve the solid waste of TBM from the seafood manufacturing factories and transformed it into valuable products in the food industry.

Graphic abstract

中文翻译：

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利用蛋白酶优化鱼腥草黑肌鱼蛋白水解产物（副产物）的生产参数

摘要

鱼蛋白水解物，在Sarda Orientalis的水解反应中作为金枪鱼加工废料释放到环境中黑肌肉，由于其在抗氧化作用方面的宝贵特性，可以用作食品中氮的营养来源。这项研究使用Protamex蛋白酶（内肽酶）水解金枪鱼的黑肌肉（TBM），并生产鱼蛋白水解物（FPH）以生产营养食品粉。为了获得FPH的产量，通过Box-Behnken模型的析因实验设计确定了水解TBM的最佳反应条件，其中酶/底物的比例为0.45％，添加的水含量为100％，温度为56°C，持续4.0 h。因此，在最佳条件下获得的FPH响应于5-20％的要求范围，平均水解度为7.72％。此外，^-1。该研究提供了一种有效的程序，用于解决海鲜制造工厂中TBM的固体废物，并将其转化为食品工业中的有价值的产品。

图形摘要