Soybean is one of the most important commodities in the world, being applied in feed crops and food, pharmaceutical industries in different ways. Soy is rich in isoflavones that in aglycone forms have exhibited significant anti-obesity and anti-lipogenic effects. Obesity is a global problem as several diseases have been related to this worldwide epidemic. The aim of this work was to verify the effect of free and immobilized β-glucosidase, testing Lentikats, and sol–gel as carriers. Moreover, we wanted to examine if the different types of hydrolysis would generate extracts with distinct biological activity concerning lipid accumulation, PPAR-α regulation, and TNF-α, IL-6, and IL-10 concentrations using in vitro assays. Our results show that all formulations of β-glucosidase could hydrolyze soy isoflavones. Thus, after 24 h of incubation, daidzein content increased 2.6-, 10.8-, and 12.2-fold; and genistein content increased 11.7, 11.4, and 11.4 times with the use of free enzyme, Lentikats®, and sol–gel immobilized enzyme, respectively. Moreover, both methodologies for enzyme immobilization led to promising forms of biocatalysts for application in the production of soy extracts rich in isoflavones aglycones, which are expected to bring about health benefits. A mild lipogenic effect was observed for some concentrations of extracts, as well as a slight inhibition in PPAR-α expression, although no significant differences were noticeable in the cytokines TNF-α, IL-10, and IL-6 as compared with the control.

大豆是世界上最重要的商品之一，以不同的方式应用于饲料作物以及食品、制药行业。大豆富含异黄酮，其苷元形式具有显着的抗肥胖和抗脂肪生成作用。肥胖是一个全球性问题，因为多种疾病与这一全球性流行病有关。这项工作的目的是验证游离和固定化 β-葡萄糖苷酶的效果，测试 Lentikats 和溶胶-凝胶作为载体。此外，我们想使用体外测定来检查不同类型的水解是否会产生具有不同生物活性的提取物，涉及脂质积累、PPAR-α 调节以及 TNF-α、IL-6 和 IL-10 浓度。我们的结果表明，所有β-葡萄糖苷酶制剂都可以水解大豆异黄酮。因此，孵育24小时后，大豆苷元含量增加了2.6倍、10.8倍和12.2倍；使用游离酶、Lentikats® 和溶胶-凝胶固定化酶后，金雀异黄素和染料木黄酮含量分别增加了 11.7 倍、11.4 倍和 11.4 倍。此外，这两种酶固定化方法都产生了有前途的生物催化剂形式，可用于生产富含异黄酮苷元的大豆提取物，预计将带来健康益处。尽管与对照相比，细胞因子 TNF-α、IL-10 和 IL-6 没有显着差异，但某些浓度的提取物具有轻微的脂肪生成作用，并且轻微抑制 PPAR-α 表达。 。