Celiac disease is an intestinal chronic disorder with multifactorial etiology resulting in small intestinal mucosal injuries and malabsorption. In genetically predisposed individuals with HLA DQ2/DQ8 molecules, the gluten domains rich in glutamine and proline present gluten domains to gluten reactive CD4+ T cells causing injury to the intestine. In the present experimental design, the indigenous bacteria from wheat samples were studied for their gluten hydrolyzing functionality. Proteolytic activity of Bacillus spp. was confirmed spectrophotometrically and studied extensively on gliadin-derived synthetic enzymatic substrates, natural gliadin mixture, and synthetic highly immunogenic 33-mer peptide. The degradation of 33-mer peptide and the cleavage specificities of the selected isolates were analyzed by tandem mass spectrometry. The gluten content of the sourdough fermented by the chosen bacterial isolates was determined by R5 antibody based competitive ELISA. All the tested isolates efficiently hydrolyzed Z-YPQ-pNA, Z-QQP-pNA, Z-PPF-pNA, and Z-PFP-pNA and also cleaved 33-mer immunogenic peptide extensively. The gluten content of wheat sourdough was found to be below 110 mg/kg. It has been inferred that four Bacillus spp especially GS 188 could be useful in developing gluten-reduced wheat food product for celiac disease prone individuals.

中文翻译：

---

从酸面团中分离出芽孢杆菌属的麸质水解活性。


乳糜泻是一种肠道慢性疾病，具有多因素病因，导致小肠粘膜损伤和吸收不良。在具有 HLA DQ2/DQ8 分子的遗传倾向个体中，富含谷氨酰胺和脯氨酸的麸质结构域将麸质结构域呈现给麸质反应性 CD4+ T 细胞，从而导致肠道损伤。在本实验设计中，研究了小麦样品中的本土细菌的麸质水解功能。芽孢杆菌属的蛋白水解活性。经分光光度法证实，并对麦醇溶蛋白衍生的合成酶底物、天然麦醇溶蛋白混合物和合成的高免疫原性 33 聚体肽进行了广泛研究。通过串联质谱分析 33 聚体肽的降解和所选分离株的切割特异性。通过基于 R5 抗体的竞争 ELISA 测定由所选细菌分离株发酵的酸面团的麸质含量。所有测试的分离株均能有效水解 Z-YPQ-pNA、Z-QQP-pNA、Z-PPF-pNA 和 Z-PFP-pNA，并广泛裂解 33 聚体免疫原性肽。小麦酸面团的面筋含量低于 110 毫克/千克。据推测，四种芽孢杆菌属，尤其是 GS 188 可用于为乳糜泻易感人群开发低麸质小麦食品。