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Toward High-Throughput Fungal Electroculturomics and New Omics Methodologies in 21st-Century Microbiology and Ecology.
OMICS: A Journal of Integrative Biology ( IF 2.2 ) Pub Date : 2020-08-03 , DOI: 10.1089/omi.2020.0012
Andreas Stathoulias 1 , Aphroditi Milioni 2 , Stavroula Kritikou 2 , Aikaterini Karmakolia 3 , Stavroula Goudoudaki 3 , Stavroula Siamoglou 1 , Constantinos Chassomeris 1 , Stamatis Vassilakis 1 , Kariofyllis Karamperis 1 , Aristea Velegraki 2 , Cleo Anastassopoulou 2 , Yiannis Manoussopoulos 3 , George P Patrinos 1 , Manousos E Kambouris 1
Affiliation  

Modern microbiology and drug development are in a watershed moment with the advent of electroceuticals. In addition to genomics, electrical impulses in an organism are believed to contribute to tissue and cellular plasticity. Hence, electroceuticals or bioelectronics offers the promise to identify innovative approaches to treat human diseases. However, applications toward electromicrobiology are still limited and rare, despite the high potential to innovate the fields of both microbiology and therapeutics. For example, electric modalities for manipulating microbial growth are highly sustainable; can be combined with biopharmaceuticals, probiotics, and pharmacobiotics; and, thus, are well poised for use in medicine, public health, and ecology and diverse industries. We report here the introduction of a new research framework and technology platform for electroculturomics, by coupling standard solid-state mycological cultures with conductive treatment using a conformité Européene (CE-)-certified medical ionophoresis device. We share our experience with a diverse range of fungi that have been treated with the electroculturomics approach reported herein. We suggest that this line of inquiry can be extended to electrotranscriptomics and electrometabolomics by deploying electroculturomics in tandem with multi-omics approaches in the future. This article makes a specific contribution to fungal microbiology, and a broader contribution to advance the theory and practice of the field of electroculturomics emerging in 21st-century microbiology and ecology research.

中文翻译:

迈向 21 世纪微生物学和生态学中的高通量真菌电培养组学和新组学方法。

随着电子药物的出现,现代微生物学和药物开发正处于分水岭时刻。除了基因组学,生物体中的电脉冲被认为有助于组织和细胞的可塑性。因此,电子学或生物电子学提供了确定治疗人类疾病的创新方法的希望。然而,尽管微生物学和治疗学领域的创新潜力很大,但电微生物学的应用仍然有限且罕见。例如,控制微生物生长的电动方式是高度可持续的;可与生物制药、益生菌、药生素联合使用;因此,它们已准备好用于医学、公共卫生、生态学和各种行业。我们在此报告引入了新的电培养组学研究框架和技术平台,通过使用符合欧洲 (CE-) 认证的医学离子导入设备将标准固态真菌培养与导电处理相结合。我们与本文报道的电培养组学方法处理过的各种真菌分享我们的经验。我们建议通过在未来将电培养组学与多组学方法结合使用,可以将这一研究范围扩展到电转录组学和电代谢组学。本文对真菌微生物学做出了具体贡献,并为推进 21 世纪微生物学和生态学研究中新兴的电培养组学领域的理论和实践做出了更广泛的贡献。通过使用符合欧洲 (CE-) 认证的医用离子导入设备将标准固态真菌培养与导电处理相结合。我们与本文报道的电培养组学方法处理过的各种真菌分享我们的经验。我们建议通过在未来将电培养组学与多组学方法结合使用,可以将这一研究范围扩展到电转录组学和电代谢组学。本文对真菌微生物学做出了具体贡献,并为推进 21 世纪微生物学和生态学研究中新兴的电培养组学领域的理论和实践做出了更广泛的贡献。通过使用符合欧洲 (CE-) 认证的医用离子导入设备将标准固态真菌培养与导电处理相结合。我们与本文报道的电培养组学方法处理过的各种真菌分享我们的经验。我们建议通过在未来将电培养组学与多组学方法结合使用,可以将这一研究范围扩展到电转录组学和电代谢组学。本文对真菌微生物学做出了具体贡献,并为推进 21 世纪微生物学和生态学研究中新兴的电培养组学领域的理论和实践做出了更广泛的贡献。我们与各种用本文报道的电培养组学方法处理过的真菌分享我们的经验。我们建议通过在未来将电培养组学与多组学方法结合使用,可以将这一研究范围扩展到电转录组学和电代谢组学。本文对真菌微生物学做出了具体贡献,并为推进 21 世纪微生物学和生态学研究中新兴的电培养组学领域的理论和实践做出了更广泛的贡献。我们与各种用本文报道的电培养组学方法处理过的真菌分享我们的经验。我们建议通过在未来将电培养组学与多组学方法结合使用,可以将这一研究范围扩展到电转录组学和电代谢组学。本文对真菌微生物学做出了具体贡献,并为推进 21 世纪微生物学和生态学研究中新兴的电培养组学领域的理论和实践做出了更广泛的贡献。
更新日期:2020-08-06
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