Early and highly accurate detection of diverse diseases is in urgent demand than ever, especially for cancers and infectious ones. Among possibilities, biosensing by utilizing conjugated nanoparticles is still a method of choice. However, the toxicity of quantum dots remains a big matter of concern in those biooriented applications. In this study, mercaptosuccinic acid-coated cadmium selenide quantum dots of approximately 2.3 nm were synthesized with a simple green method at low temperature and cost-saving chemicals. The influence of synthesis factors was investigated with different spectroscopic methods. The toxicity issue was evaluated on the NIH-3T3 cell line (ATCC® CRL-1658™) and an MTT assay, revealing a secure threshold of 20 μg/ml. Consequently, successful conjugation to the CD3 antibody including an A/G protein bridge was implemented and verified with fluorescent methods. Finally, Jurkat T cell detectability of conjugated CdSe was successfully validated with fluorescent microscopy. The CdSe-based products are accessible for future biosensing applications.

中文翻译：

---

低温合成镉量子点的Jurkat T细胞可检测性和毒性评估

与以往相比，迫切需要对各种疾病进行早期，高精度的检测，尤其是对于癌症和传染性疾病。在多种可能性中，通过利用共轭纳米颗粒进行生物传感仍然是一种选择方法。然而，在那些生物定向的应用中，量子点的毒性仍然是一个值得关注的大问题。在这项研究中，用简单的绿色方法在低温和节省化学药品的条件下合成了巯基琥珀酸涂层的硒化镉硒化量子点约2.3 nm。用不同的光谱方法研究了合成因子的影响。的毒性问题被在NIH-3T3细胞系进行评估（ATCC®CRL-1658™）和MTT测定法，揭示20的安全阈值 μ克/毫升 因此，成功实现了与包含A / G蛋白桥的CD3抗体的偶联，并通过荧光方法进行了验证。最后，通过荧光显微镜成功验证了缀合的CdSe的Jurkat T细胞可检测性。基于CdSe的产品可用于将来的生物传感应用。