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Assessment of Robustness to Temperature in a Negative Feedback Loop and a Feedforward Loop.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-06-11 , DOI: 10.1021/acssynbio.0c00023
Abhilash Patel 1 , Richard M Murray 2 , Shaunak Sen 1
Affiliation  

Robustness to temperature variation is an important specification in biomolecular circuit design. While the cancellation of parametric temperature dependencies has been shown to improve the temperature robustness of the period in a synthetic oscillator design, the performance of other biomolecular circuit designs in different temperature conditions is relatively unclear. Using a combination of experimental measurements and mathematical models, we assessed the temperature robustness of two biomolecular circuit motifs—a negative feedback loop and a feedforward loop. We found that the measured responses of both the circuits changed with temperature, both in the amplitude and in the transient response. We also found that, in addition to the cancellation of parametric temperature dependencies, certain parameter regimes could facilitate the temperature robustness of the negative feedback loop, although at a performance cost. We discuss these parameter regimes in the context of the measured data for the negative feedback loop. These results should help develop a framework for assessing and designing temperature robustness in biomolecular circuits.

中文翻译:

评估负反馈回路和前馈回路中温度的稳健性。

温度变化的稳健性是生物分子电路设计中的重要规范。虽然在合成振荡器设计中已经显示出消除参数温度依赖性可以提高周期的温度稳定性,但是相对其他不清楚的是在不同温度条件下其他生物分子电路设计的性能。通过结合实验测量结果和数学模型,我们评估了两个生物分子电路图案的温度鲁棒性-负反馈回路和前馈回路。我们发现,两个电路的测量响应都随温度变化,幅度和瞬态响应均随温度变化。我们还发现,除了取消参数温度依赖性外,尽管以性能为代价,某些参数范围可能有助于负反馈环路的温度稳健性。我们在负反馈回路的测量数据中讨论这些参数方案。这些结果应有助于建立一个评估和设计生物分子电路中温度稳定性的框架。
更新日期:2020-07-17
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