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Synthetic Glycobiology: Parts, Systems, and Applications.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-06-11 , DOI: 10.1021/acssynbio.0c00210
Weston Kightlinger 1, 2 , Katherine F Warfel 1, 2 , Matthew P DeLisa 3, 4, 5 , Michael C Jewett 1, 2
Affiliation  

Protein glycosylation, the attachment of sugars to amino acid side chains, can endow proteins with a wide variety of properties of great interest to the engineering biology community. However, natural glycosylation systems are limited in the diversity of glycoproteins they can synthesize, the scale at which they can be harnessed for biotechnology, and the homogeneity of glycoprotein structures they can produce. Here we provide an overview of the emerging field of synthetic glycobiology, the application of synthetic biology tools and design principles to better understand and engineer glycosylation. Specifically, we focus on how the biosynthetic and analytical tools of synthetic biology have been used to redesign glycosylation systems to obtain defined glycosylation structures on proteins for diverse applications in medicine, materials, and diagnostics. We review the key biological parts available to synthetic biologists interested in engineering glycoproteins to solve compelling problems in glycoscience, describe recent efforts to construct synthetic glycoprotein synthesis systems, and outline exemplary applications as well as new opportunities in this emerging space.

中文翻译:


合成糖生物学:部件、系统和应用。



蛋白质糖基化,即糖与氨基酸侧链的连接,可以赋予蛋白质工程生物学界非常感兴趣的多种特性。然而,天然糖基化系统在它们可以合成的糖蛋白的多样性、它们可以用于生物技术的规模以及它们可以产生的糖蛋白结构的同质性方面受到限制。在这里,我们概述了合成糖生物学这一新兴领域、合成生物学工具的应用和设计原理,以更好地理解和设计糖基化。具体来说,我们重点关注如何使用合成生物学的生物合成和分析工具来重新设计糖基化系统,以获得蛋白质上确定的糖基化结构,以用于医学、材料和诊断学的各种应用。我们回顾了对工程糖蛋白感兴趣的合成生物学家可用的关键生物学部分,以解决糖科学中引人注目的问题,描述了最近构建合成糖蛋白合成系统的努力,并概述了这一新兴领域的示例性应用以及新机遇。
更新日期:2020-07-17
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