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Enhanced Colorimetric Differentiation between Staphylococcus aureus and Pseudomonas aeruginosa Using a Shape-Encoded Sensor Hydrogel
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-12 , DOI: 10.1021/acsabm.0c00403 Zhiyuan Jia 1 , Lauren Gwynne 2 , Adam C Sedgwick 3 , Mareike Müller 1 , George T Williams 2 , Andrew Toby A Jenkins 2 , Tony D James 2 , Holger Schönherr 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-12 , DOI: 10.1021/acsabm.0c00403 Zhiyuan Jia 1 , Lauren Gwynne 2 , Adam C Sedgwick 3 , Mareike Müller 1 , George T Williams 2 , Andrew Toby A Jenkins 2 , Tony D James 2 , Holger Schönherr 1
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Herein, we demonstrate a combined fluorescent probe/shape-encoded hydrogel strategy for the fast, sensitive, and selective detection of bacterial species via their characteristic enzymes. A poly(vinyl alcohol) (PVA) hydrogel loaded with the fluorescent probe N,N′-(3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl)bis(2,2,3,3,3-pentafluoropropanamide) (ACS-HNE) was designed for the detection of elastase, an enzyme produced by Pseudomonas aeruginosa. Likewise, a chitosan-derived hydrogel was loaded with the fluorescent probe 4-methylumbelliferyl-α-d-glucopyranoside (MUD) by entrapment for the selective detection of α-glucosidase, an enzyme produced by Staphylococcus aureus. For an observation time of 60 min, limits of detection (LODs) of ≤20 nM for elastase and ≤30 pM for α-glucosidase were obtained, which in the latter case is 3 orders of magnitude better than related chitosan systems with covalently coupled substrate. To illustrate the potential utility of these highly sensitive sensor hydrogels as a simple point-of-care test system, shaped hydrogel slabs representing the letters P and S were manufactured to detect P. aeruginosa and S. aureus, respectively. These shapes were shown to provide an additional unique color code under UV illumination corresponding to the characteristic enzyme produced by the corresponding bacteria. This study shows potential for the future development of an effective and simple point-of-care test for the rapid identification of bacterial species that can be operated by nonspecialists.
中文翻译：
使用形状编码传感器水凝胶增强金黄色葡萄球菌和铜绿假单胞菌之间的比色区分
在这里，我们展示了一种组合的荧光探针/形状编码水凝胶策略，用于通过其特征酶快速、灵敏和选择性地检测细菌物种。负载有荧光探针N , N '-(3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(2)的聚 (乙烯醇) (PVA) 水凝胶,2,3,3,3-五氟丙酰胺 (ACS-HNE) 设计用于检测由铜绿假单胞菌产生的弹性蛋白酶。同样，壳聚糖衍生的水凝胶通过包埋法装载荧光探针 4-甲基伞形酮基-α- d-吡喃葡萄糖苷 (MUD)，用于选择性检测由金黄色葡萄球菌产生的酶 α-葡萄糖苷酶. 对于 60 分钟的观察时间，获得了 ≤20 nM 的弹性蛋白酶和 ≤30 pM 的 α-葡萄糖苷酶的检测限 (LOD)，在后一种情况下，这比具有共价偶联底物的相关壳聚糖系统好 3 个数量级. 为了说明这些高灵敏度传感器水凝胶作为简单的即时检测系统的潜在用途，制造了代表字母 P 和 S 的成形水凝胶板来检测铜绿假单胞菌和金黄色葡萄球菌， 分别。这些形状显示在紫外线照射下提供额外的独特颜色代码，对应于相应细菌产生的特征酶。这项研究显示了未来开发一种有效且简单的床旁测试的潜力，用于快速识别可由非专业人员操作的细菌种类。
更新日期：2020-07-20
中文翻译：
使用形状编码传感器水凝胶增强金黄色葡萄球菌和铜绿假单胞菌之间的比色区分
在这里，我们展示了一种组合的荧光探针/形状编码水凝胶策略，用于通过其特征酶快速、灵敏和选择性地检测细菌物种。负载有荧光探针N , N '-(3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(2)的聚 (乙烯醇) (PVA) 水凝胶,2,3,3,3-五氟丙酰胺 (ACS-HNE) 设计用于检测由铜绿假单胞菌产生的弹性蛋白酶。同样，壳聚糖衍生的水凝胶通过包埋法装载荧光探针 4-甲基伞形酮基-α- d-吡喃葡萄糖苷 (MUD)，用于选择性检测由金黄色葡萄球菌产生的酶 α-葡萄糖苷酶. 对于 60 分钟的观察时间，获得了 ≤20 nM 的弹性蛋白酶和 ≤30 pM 的 α-葡萄糖苷酶的检测限 (LOD)，在后一种情况下，这比具有共价偶联底物的相关壳聚糖系统好 3 个数量级. 为了说明这些高灵敏度传感器水凝胶作为简单的即时检测系统的潜在用途，制造了代表字母 P 和 S 的成形水凝胶板来检测铜绿假单胞菌和金黄色葡萄球菌， 分别。这些形状显示在紫外线照射下提供额外的独特颜色代码，对应于相应细菌产生的特征酶。这项研究显示了未来开发一种有效且简单的床旁测试的潜力，用于快速识别可由非专业人员操作的细菌种类。
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