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Highly Sensitive Protein Detection by Asymmetric Mach–Zehnder Interferometry for Biosensing Applications
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-11 , DOI: 10.1021/acsabm.0c00491 Melissa J Goodwin 1 , Geert A J Besselink 2 , Floris Falke 2 , Arnoud S Everhardt 2 , Jeroen J L M Cornelissen 1 , Jurriaan Huskens 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-11 , DOI: 10.1021/acsabm.0c00491 Melissa J Goodwin 1 , Geert A J Besselink 2 , Floris Falke 2 , Arnoud S Everhardt 2 , Jeroen J L M Cornelissen 1 , Jurriaan Huskens 1
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The sensitivity and performance of an asymmetric Mach–Zehnder interferometer (aMZI) were compared to those of quartz crystal microbalance with dissipation (QCM-D). The binding of streptavidin to sensor chips coated with poly-l-lysine (PLL), modified with biotin and oligoethyleneglycol (OEG) (PLL-biotin), was used to compare the binding signals obtained from both technologies. PLL-biotin proved to be an efficient method to add bioreceptors to both the QCM-D and aMZI chips. The final, saturated value of streptavidin binding was compared with those from aMZI (253 ng cm–2) and QCM-D (460 ng cm–2). These values were then used to evaluate that 45% of the measured streptavidin mass in the QCM-D came from hydrodynamically coupled water. Importantly, the signal-to-noise ratio of the aMZI was found to be 200 times higher than that of the QCM-D. These results indicate the potential of the aMZI platform for highly sensitive and accurate biosensing applications.
中文翻译：
非对称马赫-曾德干涉法用于生物传感应用的高灵敏度蛋白质检测
将非对称 Mach-Zehnder 干涉仪 (aMZI) 的灵敏度和性能与耗散石英晶体微量天平 (QCM-D) 的灵敏度和性能进行了比较。链霉亲和素与涂有聚赖氨酸 (PLL)、生物素和低聚乙二醇 (OEG) (PLL-生物素) 修饰的传感器芯片的结合用于比较从两种技术获得的结合信号。PLL-生物素被证明是一种将生物受体添加到 QCM-D 和 aMZI 芯片的有效方法。将链霉亲和素结合的最终饱和值与 aMZI (253 ng cm –2 ) 和 QCM-D (460 ng cm –2）。然后使用这些值来评估 QCM-D 中测得的链霉亲和素质量的 45% 来自流体动力学耦合的水。重要的是，发现 aMZI 的信噪比比 QCM-D 高 200 倍。这些结果表明 aMZI 平台在高度敏感和准确的生物传感应用中的潜力。
更新日期：2020-07-20
中文翻译：
非对称马赫-曾德干涉法用于生物传感应用的高灵敏度蛋白质检测
将非对称 Mach-Zehnder 干涉仪 (aMZI) 的灵敏度和性能与耗散石英晶体微量天平 (QCM-D) 的灵敏度和性能进行了比较。链霉亲和素与涂有聚赖氨酸 (PLL)、生物素和低聚乙二醇 (OEG) (PLL-生物素) 修饰的传感器芯片的结合用于比较从两种技术获得的结合信号。PLL-生物素被证明是一种将生物受体添加到 QCM-D 和 aMZI 芯片的有效方法。将链霉亲和素结合的最终饱和值与 aMZI (253 ng cm –2 ) 和 QCM-D (460 ng cm –2）。然后使用这些值来评估 QCM-D 中测得的链霉亲和素质量的 45% 来自流体动力学耦合的水。重要的是，发现 aMZI 的信噪比比 QCM-D 高 200 倍。这些结果表明 aMZI 平台在高度敏感和准确的生物传感应用中的潜力。
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