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Antifungal activity of dimethyl sulfoxide against Botrytis cinerea and phytotoxicity on tomato and lettuce plants
Plant Biosystems ( IF 1.6 ) Pub Date : 2020-06-26 , DOI: 10.1080/11263504.2020.1779846
V. Petruccelli 1 , E. Brasili 1 , L. Varone 1 , A. Valletta 1 , G. Pasqua 1
Affiliation  

For the first time the antifungal activity of dimethyl sulfoxide (DMSO) was evaluated against Botrytis cinerea, that it is one of the phytopathogenic fungi which causes the greatest damage in agriculture. In in-vitro tests, the greatest inhibitory effect of DMSO on fungal grow was recorded at pH 6. A significant growth inhibition was caused by 0.5% DMSO at 96 h post-inoculation. With higher DMSO concentrations, significant effects were recorded starting from 48 h post-inoculation. As the medium pH decreased, the inhibitory effect of DMSO also decreased. At pH 4 and 5 significant growth inhibition was caused by 1% DMSO starting from 72 h post-inoculation. At all tested pH values, a total growth inhibition was caused by ≥ 2% DMSO. On tomato leaves infected with B. cinerea, 2% DMSO significantly decreased the extent of damaged leaf area. The administration of DMSO at concentrations ranging from 0.5 to 2% through nebulization on leaves of young Solanum lycopersicum and Lactuca sativa plants did not change the chlorophyll fluorescence (Fv/Fm and ΦPSII) at any of the experimental times. Overall, the data obtained suggest that, at the concentrations tested, DMSO is toxic to B. cinerea, while it is well tolerated by lettuce and tomato plants.



中文翻译:

二甲基亚砜对灰葡萄孢的抗真菌活性及对番茄和莴苣植物的植物毒性

首次评估了二甲基亚砜(DMSO)对灰葡萄孢的抗真菌活性,它是一种在农业上造成最大损害的植物病原真菌。在体外试验中,在pH 6下记录了DMSO对真菌生长的最大抑制作用。接种后96小时,0.5%DMSO引起了显着的生长抑制。DMSO浓度较高时,从接种后48小时开始记录到明显的效果。随着培养基pH的降低,DMSO的抑制作用也降低。在接种后72小时,pH为4和5时,由1%DMSO引起明显的生长抑制。在所有测试的pH值下,总生长抑制是由≥2%DMS​​O引起的。在番茄芽孢杆菌感染,2%DMS​​O可显着降低叶片受损面积的程度。在任何实验时间,通过对年轻的茄果莴苣植物的叶片进行雾化,以0.5%至2%的浓度施用DMSO不会改变叶绿素荧光(Fv / Fm和ΦPSII)。总体而言,获得的数据表明,在所测试的浓度下,DMSO对灰质芽孢杆菌具有毒性,而生菜和番茄植物对DMSO的耐受性很好。

更新日期:2020-07-10
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