ABSTRACT

Routine preparation of paraffin embedded tissue for histopathological diagnosis, here termed conventional histological technique (CT), whether performed manually or using an automated system, requires approximately 12 h. We developed earlier a rapid acetone dehydration technique (AT) for processing biopsies of nervous tissue that meets requirements for preserving tissue morphology and staining properties, and reduces processing time to 3.3 h. We compared the morphology and staining properties of human organ biopsies including adrenal gland, liver, ovary, pancreas, prostate, testis and thyroid prepared using both AT and CT. Following fixation with 10% formaldehyde and processing by either AT or CT, sections were stained using routine and special staining, and immunohistochemical methods. We evaluated nuclear and cytoplasmic staining, staining intensity, sharpness of images and presence of artifacts such as cracking and folding. AT preserved the morphology and staining properties of the tissues as well as CT. Consequently, the rapid AT procedure is a promising alternative technique for tissue processing.

摘要

用于组织病理学诊断的石蜡包埋组织的常规制备，这里称为常规组织学技术 (CT)，无论是手动执行还是使用自动化系统，都需要大约 12 小时。我们较早开发了一种快速丙酮脱水技术 (AT)，用于处理神经组织活检，满足保留组织形态和染色特性的要求，并将处理时间缩短至 3.3 小时。我们比较了使用 AT 和 CT 制备的人体器官活检的形态和染色特性，包括肾上腺、肝脏、卵巢、胰腺、前列腺、睾丸和甲状腺。用 10% 甲醛固定并通过 AT 或 CT 处理后，使用常规和特殊染色以及免疫组织化学方法对切片进行染色。我们评估了细胞核和细胞质染色，染色强度、图像的清晰度以及是否存在裂缝和折叠等伪影。AT 保留了组织的形态和染色特性以及 CT。因此，快速 AT 程序是一种有前途的组织处理替代技术。