A multi-parametric approach to remove the influence of plasma volume on the athlete biological passport during a Union Cycliste Internationale cycling stage race.,Drug Testing and Analysis

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A multi-parametric approach to remove the influence of plasma volume on the athlete biological passport during a Union Cycliste Internationale cycling stage race.
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2020-07-02 , DOI: 10.1002/dta.2878
Laura A Garvican-Lewis _{1,

2} , Louisa M Lobigs ₂ , Tristan Equey ₃ , Catrin Goebel ₄ , Vanessa Agon ₄ , Andrew McCowan ₅ , Naomi Speers ₅ , Yorck O Schumacher ₆

Affiliation

Fluctuations in plasma volume (PV) present potential confounders within the concentration‐based markers of the haematological athlete biological passport (ABP). Here, a multi‐parametric approach involving a simple blood test is applied to the current ABP adaptive model in an attempt to remove the influence of PV expansion, induced by a cycling stage race. Blood samples were obtained from 29 professional cyclists (14 male, 15 female) before, during and after 4–5 consecutive days of racing. Whole blood was analysed in accordance with the World Anti‐Doping Agency ABP guidelines for haemoglobin ([Hb]) concentration and platelets. Serum and plasma were analysed for transferrin, albumin, calcium, creatinine, total protein and low‐density lipoprotein. PV variation (Z‐scores) was estimated using a multi‐parametric model (consisting of the biomarkers mentioned earlier) and compared against calculated variations in PV (measured via CO‐rebreathing). Significant reductions in [Hb] and the OFF‐score were observed in female cyclists after 3 and 4 days of racing, with accompanying increases in PV, which returned to baseline values 4 days post competition. Similarly, a significant increase in PV was observed in male cyclists after 3 and 5 days of racing. When individual estimations of PV variance were applied to the adaptive model, the upper and lower reference predictions for [Hb] and the OFF‐score were refined such that all outliers consistent with racing‐induced PV changes were removed. The PV model appears capable of reducing the influence of PV on concentration‐dependent markers during competition. This is an important step towards the inclusion of the PV correction in the ABP haematological module.

中文翻译：

在国际自行车联盟自行车赛阶段消除血浆体积对运动员生物护照影响的多参数方法。

血浆容量 (PV) 的波动在血液学运动员生物护照 (ABP) 的基于浓度的标记中存在潜在的混杂因素。在这里，将涉及简单血液测试的多参数方法应用于当前的 ABP 自适应模型，试图消除由自行车赛段引起的 PV 膨胀的影响。在连续 4-5 天的比赛之前、期间和之后，从 29 名职业自行车手（14 名男性，15 名女性）采集血液样本。根据世界反兴奋剂机构 ABP 的血红蛋白 ([Hb]) 浓度和血小板指南分析全血。分析血清和血浆的转铁蛋白、白蛋白、钙、肌酐、总蛋白和低密度脂蛋白。PV 变化 ( Z-分数）使用多参数模型（由前面提到的生物标志物组成）进行估计，并与计算的 PV 变化（通过 CO 再呼吸测量）进行比较。在比赛 3 天和 4 天后，女性自行车手的 [Hb] 和 OFF-score 显着降低，伴随着 PV 的增加，在比赛后 4 天恢复到基线值。同样，在比赛 3 天和 5 天后，男性自行车手的 PV 显着增加。当个体对 PV 方差的估计应用于自适应模型时，[Hb] 和 OFF 分数的上限和下限参考预测被改进，以便删除所有与赛车引起的 PV 变化一致的异常值。PV 模型似乎能够减少 PV 对比赛期间浓度依赖标记的影响。

更新日期：2020-07-02

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