Fungal mycelia can eliminate almost all cocultured cyanobacterial cells within a short time. However, molecular mechanisms of algicidal fungi are poorly understood. In this study, a time‐course transcriptomic analysis of algicidal fungus Bjerkandera adusta T1 was applied to investigate gene expression and regulation. A total of 132, 300, 422, and 823 differentially expressed genes (DEGs) were identified at 6, 12, 24, and 48 hr, respectively. Most DEGs exhibited high endopeptidase activity, cellulose catabolic process, and transmembrane transporter activity by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Many decomposition genes encoding endopeptidases were induced a little later in B. adusta T1 when compared with previously investigated algicidal fungus Trametes versicolor F21a. Besides, the accumulated expression of Polysaccharide lyases8 (PL8) gene with peptidoglycan and alginate decomposition abilities was greatly delayed in B. adusta T1 relative to T. versicolor F21a. It was implied that endopeptidases and enzymes of PL8 might be responsible for the strong algicidal ability of B. adusta T1 as well as T. versicolor F21a.

中文翻译：

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深入了解藻类真菌Bjerkandera adusta和Trametes versicolor去除蓝藻的机制。

真菌菌丝体可以在短时间内消除几乎所有共培养的蓝细菌细胞。但是，人们对杀真菌真菌的分子机制了解甚少。在这项研究中，按时程转录组学分析的杀菌剂真菌Bjerkandera adusta T1用于研究基因表达和调控。在第6、12、24和48小时分别鉴定出总共132、300、422和823个差异表达基因（DEG）。通过使用基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）分析，大多数DEG表现出高的内肽酶活性，纤维素分解代谢过程和跨膜转运蛋白活性。Adusta B.不久后诱导了许多编码内肽酶的分解基因与以前研究的杀真菌真菌Trametes versicolor F21a相比，T1 。此外，相对于杂色小麦F21a，在嗜酸芽孢杆菌T1中，具有肽聚糖和藻酸盐分解能力的多糖裂解酶8（PL8）基因的积累表达大大延迟。暗示PL8的内肽酶和酶可能是造成印度双歧杆菌T1和云芝T21a的强杀藻能力的原因。