Surface expression of human leukocyte antigen (HLA)‐class I molecules is critical for modulating T/natural killer lymphocytes' effector functions. Among HLA molecules, HLA‐C, the most recently evolved form of class I antigens, is subjected to both transcriptional and multiple post‐transcriptional regulation mechanisms affecting its cell surface expression. Among the latter a region placed in the 3′ untranslated region of HLA‐C transcript contains the single nucleotide polymorphism (SNP) rs67384697 “G‐ins/del” that has been found to be strictly associated with surface levels of HLA‐C allomorphs because of the effect on the binding site of a microRNA (Hsa‐miR‐148a). Higher expression of HLA‐C has been proved to influence HIV‐1 infection via a better control of viremia and a slower disease progression. More importantly, the analysis of SNP rs67384697 “G‐ins/del” combined with the evaluation of the HLA‐Bw4/‐Bw6 C1/C2 supratype, as well as the killer immunoglobulin‐like receptor genetic asset, has proved to be pivotal in defining the status of Elite Controllers in the Caucasian population. Here we describe a new reliable and fast method of allele‐specific real‐time PCR to monitor the integrity/disruption of the binding site of the microRNA Hsa‐miR‐148a in a high‐throughput format that can be easily applied to studies involving large cohorts of individuals.

中文翻译：

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通过单次运行等位基因特异性实时PCR检测SNP rs67384697（Hsa-miR-148a结合位点）的快速，可靠的方法。

人类白细胞抗原（HLA）I类分子的表面表达对于调节T /天然杀伤淋巴细胞的效应子功能至关重要。在HLA分子中，HLA-C（I类抗原的最新进化形式）受到转录和多种转录后调控机制的影响，从而影响其细胞表面表达。在后者中，位于HLA-C转录本3'非翻译区的区域包含单核苷酸多态性（SNP）rs67384697“ G-ins / del”，已发现与HLA-C同种异型体的表面水平严格相关。对microRNA（Hsa-miR-148a）结合位点的影响。事实证明，HLA-C的高表达可通过更好地控制病毒血症和减缓疾病进程来影响HIV-1感染。更重要的是，SNP rs67384697“ G-ins / del”的分析与HLA-Bw4 / -Bw6 C1 / C2超型的评估以及杀伤性免疫球蛋白样受体遗传资产的结合已被证明对确定状态至关重要白种人人口中的精英管制员 在这里，我们描述了一种新的可靠，快速的等位基因特异性实时PCR方法，以高通量格式监测microRNA Hsa-miR-148a结合位点的完整性/破坏，可以轻松地应用于涉及大分子的研究个人队列。