A 6‐week study was conducted to assess the effect of dietary fishmeal (FM) replacement with Sargassum ilicifolium meal (SIM) at four substitution levels including 0 (control), 3% (S3), 6% (S6) and 9% (S9) on performance of Asian sea bass (Lates calcarifer) juveniles (initial mean body weight of 29.0 ± 1.0 g). Growth performance pronouncedly increased in SIM‐supplemented groups compared to the control (p < .05). Fish fed on the S6 diet had higher pancreatic digestive enzyme activities than other treatments. Supplementing diet with SIM remarkably enhanced red blood cell count compared to the control. Moreover, fish in the control and S3 groups had higher lymphocyte, but lower neutrophil percentages than the S6 and S9 treatments (p < .05). The greatest and the least amounts of the total protein and total immunoglobulins (Ig) in serum were found in the S6 and the control, respectively. The amount of alternative complement pathway activity in the serum of fish in the S9 group was higher than the other groups, whereas higher serum lysozyme activity was found in the S6 and S9 treatments compared to those in the control and S3 groups (p < .05). Total Ig content of the skin mucus in the S6 and S9 groups was higher than the control. The greatest and the least mucosal lysozyme activates were found in the S6 and control, respectively (p < .05). The liver superoxide dismutase activity in the S6 and S9 groups was higher than the other treatments (p < .05). The insulin‐like growth factor‐1 mRNA transcript abundance levels in the liver were greatest and the least in fish fed on the S9 and control, respectively. The expression of lysozyme gene, as indicated by an abundance of mRNA transcript for lysozyme, in the liver was higher in the S6 and S9 groups than the other groups (p < .05). Furthermore, fish fed the SIM‐incorporated diets had higher interlukine‐1β mRNA transcript abundance in the liver compared to the control (p < .05). Overall, according to the findings of this study 6% of dietary FM could be replaced with SIM to improve growth rate and health status in L. calcarifer juveniles.

中文翻译：

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用马尾藻代替鱼粉对晚cal鳞幼鱼的生长，先天免疫和免疫基因mRNA转录本丰度的影响

进行了一项为期6周的研究，以评估在4种替代水平下使用Sargassum ilicifolium餐（SIM ）替代膳食鱼粉（FM）的效果，包括0（对照），3％（S3），6％（S6）和9％（ S9）对亚洲鲈鱼（Lates calcarifer）幼鱼的表现（初始平均体重为29.0±1.0 g）。与对照组相比，SIM补充组的生长性能明显提高（p  <.05）。以S6饮食喂养的鱼比其他处理方法具有更高的胰腺消化酶活性。与对照相比，在饮食中添加SIM可以显着提高红细胞计数。此外，对照组和S3组的鱼具有更高的淋巴细胞，但中性粒细胞百分率低于S6和S9处理（p <.05）。血清中总蛋白和总免疫球蛋白（Ig）的最大和最小分别在S6和对照中。S9组鱼血清中替代补体途径活性的数量高于其他组，而S6和S9处理中的血清溶菌酶活性高于对照组和S3组（p  <.05 ）。S6和S9组中皮肤粘液的总Ig含量高于对照组。在S6和对照组中分别发现最大和最小的粘膜溶菌酶激活（p  <.05）。S6和S9组的肝超氧化物歧化酶活性高于其他治疗（p <.05）。肝脏中胰岛素样生长因子-1 mRNA转录物的丰度水平最高，而以S9和对照组为食的鱼类中最低。溶菌酶基因的表达，通过丰富mRNA转录物对于所指示的溶菌酶，在肝脏中是在S6和S9团比其它基团（较高p  <.05）。此外，与对照相比，饲喂含SIM的饲料的鱼在肝脏中具有较高的interlukine-1βmRNA转录丰度（p  <.05）。总体而言，根据这项研究的结果，可以用SIM代替6％的膳食FM，以改善钙生L. calcarifer幼鱼的生长速度和健康状况。