Transforming the future capability to protect and restore vulnerable environments hinges on the ability to monitor them in real‐time with ever‐increasing sensitivity. Combining the precision and proficiency of biomolecules to signal the presence of low‐level toxins is key to developing water system monitoring that enables fast detection and remediation of pollution. Here, a 3D flow sensor is developed incorporating enzymes which can signal the presence of organic toxins from water systems over extended periods of time. The enzymes are encapsulated in a porous, crystalline metal–organic framework (MOF) cage, providing protection and stability. The inline flow device features a cutting‐edge 3D‐printed design to maximize flow and interfacial interactions between the encapsulated organophosphate degrading enzyme (OpdA) and the organophosphate based pollutants. The OpdA produces a yellow product on decomposition of the pollution that signals its presence. The porous protective MOF coating is key to this technology, structurally securing the enzyme while allowing the flow of reactant and thus the continuous breakdown of organic pollution over several days.

中文翻译：

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制造具有生物活性的3D金属有机框架设备

未来保护和恢复脆弱环境的能力的转变取决于以越来越高的敏感性实时监视它们的能力。将生物分子的精密度和熟练度相结合以发出低水平毒素的信号，这是发展水系统监测的关键，该监测能够快速检测和修复污染。在这里，开发了一种3D流量传感器，其中包含了酶，这些酶可以在很长的一段时间内发出来自水系统的有机毒素的信号。这些酶被包封在一个多孔的晶体金属-有机框架（MOF）笼中，提供保护和稳定性。在线流量装置具有尖端的3D打印设计，可最大程度地提高封装的有机磷酸酯降解酶（OpdA）与有机磷酸酯类污染物之间的流量和界面相互作用。OpdA在污染分解时产生黄色产物，表明存在该污染物。多孔保护性MOF涂层是该技术的关键，它在结构上确保了酶的安全性，同时又允许反应物流过，从而在几天内连续分解了有机污染物。