Abstract Wound dressings made from natural polymers are an important aspect of biomaterials. Protein-based materials are less likely to instigate an immunogenic response and have the capacity to degrade in vivo, also without triggering an inflammatory response. Therefore, gelatin (GEL) was chosen and combined with bacterial cellulose (BC) to produce nanofibres and the potential of an all-natural polymer construct was determined. GEL and BC were successfully electrospun with metformin (Met) and glybenclamide (Gb) using a portable, point of need electrospinning set up. The virgin fibre group exhibited a significant effect on the proliferation of L929 (mouse fibroblast) cells but all fibre samples can safely be applied on wound site without risk of cytotoxicity. According to the results obtained by animal tests, the GEL-BC-Gb group showed better recovery than the GEL-BC-Met group. Diabetic wounds treated with GEL-BC-Met were characterized by moderate re-epithelialization and partially organized granulation tissue. Moderate to complete re-epithelialization and well-formed granulation tissue were observed in diabetic wounds treated with GEL-BC-Gb. The histologic scores obtained on day 14 confirmed that the GEL-BC-Gb group played a stronger wound-healing role compared to the GEL-BC-Met group. The highest decrease of TNF-α level was observed in the GEL-BC-Gb group at the end of the experiment but there is no significant difference between drug-loaded fibre groups. Therefore, topical administration of Met and Gb in a sustained release form has a high potential for diabetic wound healing with high bioavailability and fewer systemic side effects but Gb showed better improvement according to the results of the animal tests.

中文翻译：

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用于糖尿病伤口愈合的便携式电流体动力枪生产的甘苯脲和装载二甲双胍的细菌纤维素/明胶纳米纤维的比较

摘要 由天然聚合物制成的伤口敷料是生物材料的一个重要方面。基于蛋白质的材料不太可能引发免疫原性反应，并且能够在体内降解，而且不会引发炎症反应。因此，选择明胶 (GEL) 并与细菌纤维素 (BC) 结合生产纳米纤维，并确定了全天然聚合物结构的潜力。GEL 和 BC 与二甲双胍 (Met) 和甘苯脲 (Gb) 使用便携式、需要点的静电纺丝装置成功地进行了静电纺丝。原始纤维组对 L929（小鼠成纤维细胞）细胞的增殖表现出显着影响，但所有纤维样品都可以安全地应用于伤口部位，没有细胞毒性的风险。根据动物试验的结果，GEL-BC-Gb 组显示出比 GEL-BC-Met 组更好的恢复。用 GEL-BC-Met 治疗的糖尿病伤口的特征是中度上皮再形成和部分组织的肉芽组织。在用 GEL-BC-Gb 治疗的糖尿病伤口中观察到中度至完全的再上皮化和形成良好的肉芽组织。第 14 天获得的组织学评分证实，与 GEL-BC-Met 组相比，GEL-BC-Gb 组具有更强的伤口愈合作用。在实验结束时，在 GEL-BC-Gb 组中观察到 TNF-α 水平的最大降低，但载药纤维组之间没有显着差异。所以，