Objectives

The aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017.

Material/methods

A total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure.

Results

MALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi).

Conclusions

These results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method.

中文翻译：

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使用 MALDI-TOF MS (Bruker Daltonics) 鉴定直接从液体培养基中分离的分枝杆菌种

目标

本研究的目的是描述对使用 MALDI-TOF MS直接从 2017 年 1 月至 2017 年 12 月液体 MGIT 培养物中鉴定非结核分枝杆菌 (NTM) 和结核分枝杆菌的评估。

材料/方法

通过 MALDI-TOF MS (Bruker Daltonics) 直接从 MGIT 液体培养基中使用先前的提取程序分析了总共 155 个分离株（主要是呼吸道）。

结果

MALDI-TOF MS 为 152 个分离株 (98.06%) 生成了可接受的分数。五十分离物鉴定为结核分枝杆菌复合并且剩余105作为NTM（M.脓肿亚种脓肿，鸟分枝杆菌，M. celatum，男龟，M.嵌合体，偶发分枝杆菌，戈氏分枝杆菌，胞内分枝杆菌， M. kansasii、M.lentiflavum、M.mageritense、M. mucogenicum和M. xenopi )。

结论

这些结果表明 MALDI-TOF MS 可用于直接从 MGIT 培养物中识别分枝杆菌，并且是一种准确、快速且具有成本效益的系统，可用作常规方法。